新疆牛源奇异变形杆菌分离鉴定及耐药与毒力基因分析

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图分类号：Q78，S859 文献标志码：A 文章编号：1008-0864（2025）07-0142-0：

Isolation and Characterization of Bovine-origin Proteus mirabilis from Xinjiang and Analysis of Drug Resistanceand Virulence Genes

HEWei 1，2 ， SONG Linqing1，2，WU Xinglin1，AI Junjie12，JI Liwei³，WANG Limin 1，2 WANG Yanbin 1，2 ，PENG Bin1²，DAI Xiaohua 1，2* ， GUO Qingyong1，2*

（1.ColegeofVeterinary Medicine，XinjiangAgriculturalUniversity，Urumqi830o52，China;2.KeyLaboratoryofNewDrug

ResearchandDevelopmentforherbivoresofXinjiang，Xinjiang Agriculture University，Urumqi83oo52，China;3.Shandong Animal Husbandry and Veterinary Medicine College，Shandong Weifang 261O61，China）

Abstract：Inordertostudythepathogenicbacteriaand theircharacteristicsofendometritis inadairyfarminChangji regionof Xinjiang，conventionaland PCR methods were used to isolateand identifyProteus mirabilis inthecollected secretion samples，and K-B drug-sensitive paper slides，PCR methods and artificially infected mice were used to detect thedrug resistance，virulence genes and pathogenicityof Proteus mirabilis，respectively.The results showed that 29 strainsof Proteus mirabilis were isolated.Thedrug susceptibility testof16 drugscommonly showed that 29 strains weresensitive to9drugs，resistant to5drugs，and moderately sensitive to2 drugs.The detection rateof virulence genes，ureC，atfC，rsmA，atfA，hpmAand rsbA ，ranged from 62.07% to 89.66% ，and the detection rate of other virulence genes was from 13.79% to 41.38% .Theanimal pathogenicity test showed that the isolated strain had pathogenicity to mice.Above results provided a scientific basis for the clinical control of the disease.

Keywords：endometritis；Proteusmirabilis；drug resistance；pathogenicity

奇异变形杆菌为肠杆菌科变形杆菌属，无荚膜和芽孢但有鞭毛和菌毛，属革兰氏阴性杆菌，广泛分布于自然界及人和动物的黏膜和消化道中2。（剩余11164字）