外源基因插入位点对抗虫棉Bt蛋白含量的影响

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中图分类号：S562；Q786 文献标志码：A 文章编号：1008-0864（2025）07-0044-10

Effect of Exogenous Gene Insertion Site on Bt Protein Content in Insect-resistantCotton

ZHAO Guiyuan 1，2 ，WANG Yongqiang²，LIU Jianguang²，GENG Zhao²， ZHANG Hanshuang² WULiqiang1，WANG Xingfen1*，ZHANG Guiyin1*

（1.StateKeyLaboratoryofNorthChinaCropImprovemenandRegulation，KeyLaboratoryforCropGermplasmResourcesof Hebei，ColegeofAgronomy，Hebei AgriculturalUniversity，HebeiBaodingO7Oo，China;2.KeyLaboratoryofCottonBiology andGeneticBreedinginHuanghuaihaiSemiaridAreaofMinistryofAgricultureandRuralAfairs，InstituteofCoton，Hebei AcademyofAgricultureand forestrySciences，ShijiazhuangO5oO51，China）

Abstract：The insertion sites of exogenous genes can affct the gene expression and character expression of recipient materials.Inorder todeterminetheefectof exogenousgeneinsertionsitesonBtproteincontentof insect-resistant coton，29 insect-resistant coton lines were selected for Bt protein content determination and wholegenome resequencing analysis.The results showed thatthere were significant diferences in Bt protein content among diffrent lines，and the K3 line was the highest.Whole-genome resequencing analysis and PCR identification revealedthat the Btgene wasa single copyin 29 lines，but the insertion sites were different. The Bt gene of K3 line was located in the 2 791 303＼～2 791 335 bp of A02 chromosome，while the Bt gene of other lines were located in D12 chromosome. To further verifytheefectof insertionsiteon Btproteincontent，theK3 line was hybridization withcontrol Xinmian 33B，and F2 population was constructed. The results showed that the Bt protein content of F2 individual plants with theinsertion siteof Bt gene in AO2 chromosome was significantly higher than that D12 type individual plants.In summary，the Bt protein content was affected by the insertion site of Bt gene，and its location on chromosome A02 was more conducive to the efficient expression of Bt gene.Above results could provide germplasm resources and theoretical support for breeding new varieties of insect-resistant cotton.

Keywords：insect-resistant cotton；exogenous gene；insertion site；Bt protein

棉花（Gossypium spp.）是集纤维、油料、饲料于一身的重要经济作物。（剩余16106字）