ANKRD22在胰腺癌中的表达及对肿瘤的影响

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【中图分类号】R576；R735.9 【文献标识码】A

Expression of ANKRD22 and its effects on pancreatic cancer

SUN Zhijia， SONG Zhuo， LIU Xu， LI Xinji， WANG Yingjie DepartmentofTumorRadiotherapy，PLAAirForceMedicalCenter，Beijing10o142，China Corresponding author： WANG Yingjie， Email： wangyj9999@163.com

【Abstract】 Objective To explore the expression of ankyrin repeat domain 22 （ANKRD22） in pancreatic cancer （PC），to analyze its clinical prognosis， and investigate its impact on PC cell phenotypes. Methods The expresson levels of ANKRD22 and their impact on PC prognosis were analyzed using R and Perl languages in the TCGA-PC and GEO databases.ANKRD22-interfered PANC-1 and AsPC-1 celline models were constructed using siRNA. Cell proliferation and invasion capabilities were assessed using CCK-8 assays，colony formation，and Transwell experiments. The regulatory effect of ANKRD22 knockdown on cell apoptosis was evaluated by flow cytometry. Results Compared to normal pancreatic tissues，ANKRD22 expression was significantly elevated in PC tissues.Patients with high ANKRD22 expression had a significantly lower overall survival than those with low expresson， correlating with poor prognosis. Knockdown of ANKRD22 significantly attenuated the proliferation and invasion capabilities of PC cells but did not affect cell apoptosis. Conclusion ANKRD22 is highly expressed in PC and promotes the proliferation and invasion of PC cells. ANKRD22 may serve as a crucial therapeutic target for PC.

【Keywords】Pancreatic cancer; ANKRD22; Clinical prognosis; Cell proliferation; Cell invasion

胰腺癌（pancreaticcancer，PC）是常见的消化道肿瘤之一，随着生活水平的提高，其发病率和死亡率呈逐年上升趋势。（剩余11761字）