外源水杨酸对海南紫荆木关键防御基因及代谢通路的影响

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中图分类号：S718.3 文献标志码：A 文章编号：1001-411X（2026）01-0150-13

Abstract： 【Objective】To explore defense genes and metabolic pathways influencing post-germination growth of Madhuca hainanensis seeds. 【Method】 The changes of seed fresh weight and seedling height of M. hainanensis after exogenous salicylic acid （SA） treatment were determined. Combined with Illumina transcriptome and targeted metabolomics，correlation analysis between diferentially expressed genes and metabolic pathways was conducted. Weighted gene co-expresson network analysis （WGCNA） and validation of key defense genes were performed. 【Result】 Under exogenous SA treatment， the seed fresh weight change rate and seedling height growth rate of M. hainanensis were lower than those in the blank control group by 1.47 and1.68 percentage points， respectively. Defense-related plant hormones such as SA and jasmonic acid， and abundant secondary metabolitesaccumulated in the five-leaf-stage sedlings ofM. hainanensis.Meanwhile， numerous defense-related pathways were activated， including plant-pathogen interaction， plant hormone signal transduction，MAPK signaling pathway-plant， and biosynthesis of secondary metabolites. NFP，RIN4， PK1， WRKY17 and WRKY53 were identified as key defense genes enriched in the plant-pathogen interaction pathway. 【Conclusion】 Exogenous SA affects post-germination growth of M. hainanensis seeds，and the five-leaf-stage seedlings initiate defense mechanisms with key defense genes mainly enriched in the plant-pathogen interaction pathway. This study provides a theoretical foundation for analyzing the potential mechanisms underlying the slow growth of M. hainanensis seedlings.

Key words： Salicylic acid; Madhuca hainanensis; Growth and defense; Weighted gene co-expression network; Metabolic pathway

海南紫荆木MadhucahainanensisChun&F.C.How又称子京、海南马胡卡、铁色、刷空母树，是山榄科Sapotaceae紫荆木属Madhuca常绿乔木[]，分布于海南岛西部和南部，属国家重点保护野生植物（II级）、世界自然保护联盟（InternationalUnionforConservationofNature，IUCN）红色名录易危等级，是珍贵的用材树种和油料树种。（剩余15825字）