菠萝黑钉病病原菌分离鉴定及防控药剂筛选

打开文本图片集

中图分类号：S435.79；S471 文献标志码：A 文章编号：1001-411X（2026）01-0064-10

Abstract： 【Objective】To isolate and identify the pathogen responsible for Pineapple （Ananas comosu fruitlet core rot （FCR），and screen control agents and field-based combination treatments，thereby providing a basis for studying the disease patern and mechanism as well as green prevention and control. 【Method】 Pathogens were isolated and purified， and the pathogenicity was verified using Koch's law. The species of pathogens were determied according to the morphological characteristics of the strainsand multi-gene sequence analysis.The inhibitory effects of the pathogen on five single agents and eight compound agents were determined using the mycelial growth rate method. 【Result】Nine genera of fungal strains were obtained by isolation and purification， among which two strains could infect pineapple fruitlets and cause brown spots. Combining morphological characterization with multi-gene sequence analysis， the pathogenic fungi that caused FCR were identified as Fusarium verticilioides LDL-3 and Talaromyces funiculosus LL-3， with LDL-3 being more pathogenic than LL-3. The results of fungicide virulence tests showed that flucytosine， feniconazole， pyraclostrobin， imidacloprid manganese salts and compound formulations had effects on the growth of the pathogens. Among these agents，imidacloprid manganese salts had a better inhibitory effect on LDL-3 with an EC50 of 0.009μg/mL ，while pyraclostrobin showed a better inhibitory effect on LL-3 with an EC50 of 0.134 μg/mL . Among eight compound formulations， benzyl ·pyraclostrobin （volume ratio of difenoconazole and pyraclostrobin was 3：1 ） inhibited both pathogens effectively with EC50 lessthan 0.2μg/mL . The combined use of insecticide， fungicide and growth regulator could effectively inhibit the pathogen growth with EC50 less than 0.2μg/mL . 【Conclusion】 The FCR is mainly caused by Fusarium sp. and Talaromyces sp.. Benzyl pyraclostrobin （3：1，ν/ν） effectively inhibits these pathogens and offers a simplified and efficient solution for field control during flower induction and fruit expansion.

Key words： Ananas comosu; Fruitlet core rot; Pathogen identification; Fungicide; Virulence; Benzyl pyraclostrobin

菠萝Ananascomosu为凤梨科凤梨属，是典型的热带多年生草本植物，也是我国重要的经济作物[]。（剩余11956字）