越南蔷薇菌YJ21菌株鉴定及生防活性测定

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中图分类号：S435.111.4 文献标志码：A 文章编号：1001-411X（2026）01-0052-12

Abstract： 【Objective】To screen bacteria from mangrove soil that can inhibit the growth and development of Sporisorium scitamineum， Magnaporthe oryzae and Phytophthora capsici while promoting plant growth， thus providing biocontrol resources for managing crop diseases caused by these pathogens. 【Method】 Bacteria were isolated from mangrove soil using the dilution spread plate method. The species of bacteria was identified through 16S rRNA sequencing and whole genome sequencing. The antagonistic effects of the bacteria against phytopathogens were evaluated via plate confrontation assay，spore germination test， in vitro leaf inoculation test，and pot inoculation experiment. Salkowski colorimetry was used to determine the indole-3-acetic acid （IAA） production capacity of isolated bacterial strain，and the promotive effect of strain on rice seed germination was evaluated via the seed soaking assay. 【Result】 Strain YJ21 was isolated from the mangrove soil. It was identifiedas Rossellomorea vietnamensis through genomic evolutionary analysis.The inhibition rates of the YJ21 strain on the mycelial growth of M. oryzae and P .capsici were 34.62% and 39.87% ， respectively. Its fermentationsupernatant inhibited the teliospore germination in S⋅ scitamineum teliospores，theinhibition rate of conidial germination in M. oryzae reached 90.48% ， and the supernatant also reduced the pathogenicity of M. oryzae on rice.Furthermore，the YJ21 strain was capable of producing IAA and protease，and it significantly promoted the growth of root and shoot after rice seed germination. 【Conclusion】R. vietnamensis YJ21， isolated from mangrove soil， has a potential application for the biocontrol of rice blast.

Key words： Rossellomorea vietnamensis;Mangrove sediments; Rice blast; Biocontrol

作物病害威胁着农业生产，长期使用农药不仅会导致农药有效性下降，还会造成环境污染[1]。（剩余21037字）