基于免疫组化分析的胶质瘤分子表型与临床病理特征关联研究

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中图分类号：R739.41 文献标志码：A

Association between molecular phenotypes and clinicopathological characteristics in gliomas： an immunohistochemical analysis

ZI Hao，LIU Haoyu，WU Jiajie，JIANG Kaiyuan （Department of Neurosurgery， Shaoyang Hospital Afiliated to University of South China， Shaoyang 422000，China）

Abstract： Objective To investigate the expression patterns of immunohistochemical markers in glioma tissues and their correlation with clinicopathological parameters，providing a basis for molecular clasification and prognosis assessment.Methods Twenty-one glioma cases were collected. Immunohistochemistry was performed to detect the expression of molecular markers，including glial fibrillary acidic protein （GFAP）， isocitrate dehydrogenase-1 R132H mutant protein （IDH-1 R132H），Ki-67 antigen （Ki-67），S-100 protein （S-100），synaptophysin（Syn），and tumor protein p53（p53）.Statistical analysiswas conducted in conjunction with clinical data and WHO grading. Results The study cohort was predominantly composed of high -grade gliomas （WHO grades I～N ， 66.7% ）.Glioblastoma（9 cases， 42.9% ）was the most common subtype.The incidence was higher in patients aged 46 to 61 years （38.1%） ）and ⩾61 years （33.3%） ： Molecular marker analysis revealed high positive rates for GFAP （ 90.5% ）and S-100（ 85.7% ）. The Ki-67 proliferation index ranged from 2% to 40% . An aberrant p53 expression rate of 55.6% was observed. A Syn positivity rate of 66.7% suggested a tendency toward neuronal differentiation in some of the tumors. Among the 14 cases tested for IDH-1，13（ 92.9%） were wild-type，and only1 （7.1%） was mutant. Conclusion The gliomas in thiscohort were primarily IDH wild-type and clustered in themiddle-agedand elderly patients exhibiting features of high proliferative activity（elevated Ki-67 index）and aberrant p53 expression. This molecular profile helps define a more aggressive glioma subtype and holds referential value for its clinical diagnosis and prognosis evaluation.

Key words： glioma； immunohistochemistry；molecular classification； IDH-wildtype； Ki-67

胶质瘤是中枢神经系统最常见的原发性恶性肿瘤，具有高度的病理异质性及显著的预后差异，严重威胁患者的生存质量[1]。（剩余9697字）