转录组及代谢组关联分析解析西南草莓果实中关键生物活性物质形成机制

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Abstract：Fragaria moupinensis is a tetraploid strawberry species native to China，offering both edible and medicinal benefits.However，research on the bioactive compounds present in F. moupinesis fruits remains limited.This study combined metabolomic and transcriptomic analyses to identify key bioactive substances in F moupinensis fruit，investigate their dynamic changes during ripening，and elucidate the underlying molecular mechanisms of their biosynthesis.Metabolomic analysis revealed that pathways related to ABC transporters， phenylpropanoid biosynthesis，galactose metabolism，and flavonoid and flavonol biosynthesis are involved in the regulation of fruit ripening in F. moupinesis. And the levels of multiple bioactive flavonoids， flavonols， flavone，flavanones，and isoflavones—including morin，avicularin，rhoifolin，kaempferol-3-O-galactoside， eriocitrin，and genistein，which possessantioxidant and anti-inflammatory activities—progressively increased during fruit maturation.Transcriptomic analysis revealed that pathways related to flavonoid biosynthesis， glycolysis/gluconeogenesis，and the citrate cycle are involved in the regulation of fruit ripening in F. （ moupinensis. Integrated transcriptomic and metabolomic analyses revealed that the phenylpropanoid biosynthesis pathway plays a crucial role in the biosynthesis of these key active compounds. This research provides a theoretical foundation for enhancing the medicinal value of F. moupinensis and for the genetic improvement of cultivated strawberry varieties.

Key words： Fragaria moupinensis; transcriptomic;metabolomic ; bioactive compounds ; fruit ripening

草莓是世界上栽培最为普遍的小浆果，目前草莓属共有约25个种，其中只有八倍体凤梨草莓被广泛栽培，其余均为野生种。（剩余17574字）