基于ISSR标记的朝鲜淫羊藿种质资源遗传多样性分析

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Abstract： Epimedium koreanum Nakai，a medicinal plant of critical importance in Northeast China，has experienced severe depletion of its wild populations due to overharvesting. Investigating the genetic diversity of itsgermplasm and elucidating phylogeographic relationshipsamong populations are therefore essential for developing efective conservation strategies.The research employed ISSR（Inter simple sequence repeat） marker technology. Through preliminary optimization of the reaction system，seven highly polymorphic primers were selected for genetic diversity analysis of 128samples collcted from14 natural populations in Northeast China. Results showed that these seven ISSR primers amplified a total of 8O bands，with 74（ 92.60% ）being polymorphic.Number of observed alleles（Na）is 1.6438，effective number of alleles （ ΠNe） is 1.3747，Nei' s gene diversity index （H） is 0.2189，and Shannon's information index （I） is 0.3290. The WNS population （Wunu Mountain Scenic Area，Huanren county，Liaoning province） showed the highest genetic diversity，while the DLZC population （Dalizi villge，Linjiang city，Jilin province） exhibited the lowest. After comparison with other species in the same genus，it was found that the genetic diversity of Epimedium koreanum germplasm resources is at a moderate level. Genetic similarity coefficients among populations ranged from 0.7441 to 0.9700， with genetic distances between 0.0304 and 0.2955.Mantel test showed no significant corelation between genetic and geographical distances，suggesting that population diferentiation may be influenced by habitat isolation or reproductive characteristics rather than geographical distance. Genetic structure analysis （ Gst=0.2784 ）indicated that 72.16% of genetic variation occurred within populations. Furthermore，cluster analysis divided the 14 populations into five distinct groups.This study provides fundamental data for the conservation of genetic diversity and germplasm resource management of E .koreanum.

Key words： Epimedium koreanum Nakai; ISSR; genetic diversity; phylogenetic relationships;conservatior measures

朝鲜淫羊藿（EpimediumkoreanumNakai）是小檗科淫羊藿属多年生草本植物，被收录于2025版《中华人民共和国药典》，是中药淫羊藿的主要基原植物之一[1]。（剩余18217字）