长穗偃麦草5Ag染色体在不同小麦背景中的传递分析

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Abstract：In order to study the genetic stability of 5Ag chromosome from Thinopyrum ponticum in different wheat backgrounds and its transmission through gametes，the wheat-Th.ponticum disomic substitution line DS5Ag（5D）（Bainong Puyan 5814， PY5814） was hybridized with six wheat cultivars（lines）： Chinese Spring， Bainong Aikang 58，Lankaoaizao 8，Wenmai 6，Zhoumai 16，and Q03073.The F1 hybrids were selfed and reciprocally crossed with the six aforementioned varieties（lines），the F2 and BC1F1 generations were genotyped using molecular marker technology. Results showed that all F1 hybrids derived from PY5814 and the six wheat varieties（lines） exhibited normal seed set.In the F2 segregation populations of different crosses，the transmission rate of the 5Ag chromosome ranged from 51.61% to 71.76% ，indicating that the transmission rate of 5Ag （204 chromosome was different in different wheat genetic backgrounds，with its transmission being influenced by the wheat geneticbackground.In BC1F1 populations，the transmission rates of the 5Agchromosome through female and male gametes ranged from 21.43% to 31.73% and 29.51% to 39.73% ，respectively，confirming its transmission via both gamete types.Notably，under the genetic backgrounds of Lankaoaizao 8 and Wenmai 6， the male gamete transmission rate exceeded the female gamete rate，suggesting the preferential suitability of this substitution line as a paternal parent in genetic studies.At the same time，some F2 individualswere verified by genomic in situ hybridization （GISH），and the results were consistent with the results of molecular markers. Furthermore，this study also oers theoretical guidance for predicting and establishing an appropriate hybrid population size， thereby enabling the selection of target progenies carrying the 5Ag chromosome.

Key words： common wheat; Thinopyrum ponticum; 5Ag chromosome substitution line; female and mal gamete transmission rates

十倍体长穗偃麦草［Thinopyrumponticum（Podp.）Barkworth &D.R.Dewey， 2n=10x=70 EEEEEEStStStSt是禾本科小麦族偃麦草属（ThinopyrumLove）的多年生草本植物，原产于欧洲东南部、小亚细亚和俄罗斯南部[，含有抗白粉病、叶锈病和条锈病等多种抗病基因，是可供小麦（TriticumaestivumL. ，2n=6x=42 ，AABBDD）遗传育种利用的重要种质资源[2]。（剩余14067字）