高危型人乳头瘤病毒联合p16/Ki-67双染在宫颈鳞状上皮内病变组织学诊断中的辅助价值

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【Abstract】 Objective To explore the auxiliary value of high-risk human papilloma virus （HPV） combined with dual staining ofcyclin-dependent kinase inhibitor （p16）and proto-oncogene cellproliferation nuclear antigen （Ki-67）in the histologicaldiagnosis of cervical squamousintraepithelial lesions.MethodsAtotalof78 female patients who underwent cytological screening in the Departmentof Pathology of Luoyang Maternal and Child Health Hospital from January 2O22 to December 2O24and had cervical tissue pathological examination within1 month wereselected.Allcervical tissue specimens were tested for high-risk HPVanddual stainingofp16/Ki-67.The histopathological examination results were used as the diagnostic gold standard to compare the diagnostic results of HPV，p16/Ki-67 dual staining alone，and combined detection with the pathological examination results.Results The Kappa test results showed that the consistency between HPV detectionresultsand pathological examination results was moderate （Kappa value =0.373 ， P =0.001），the consistency between p16/Ki-67 dual staining detection and pathological examination results was moderate （Kappa value = 0.403， P<0.001 ），and the combined detection diagnosticresultshadahigh consistencywith thepathological examination diagnostic results （Kappa value =0.820 （2 P=0.000 ）.There was no significant difference in the pathological diagnosis results，HPV，and p16/Ki-67 expression among different age groups（ P>0.05 ）.The sensitivityand specificity of the combined detection of HPV and p16/Ki67 were 91.89% and 90.24% ，respectively，both of which were higher than those of single-item detection alone. ConclusionHigh-risk HPVcombined with p16/Ki-67 dual stainingcan beused asa supplementary tool for histopathologyandcan improve the diagnosticaccuracyofcervical squamous intraepithelial lesions and reducethe rateof missed diagnoses.

【Key words】 Uterine cervical dysplasia; human papiloma virus； Inhibitory factors of cyclin kinase; Proto-oncogene cell proliferation nuclear antigenDOI：10.19522/j.cnki.1671-5098.2025.11.013

宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）是宫颈癌前病变，其发生与高危型人乳头瘤病毒（humanpa-pil-lomavirus，HPV）持续感染密切相关。（剩余5696字）