天麻素通过调控外泌体IncRNAMeg3/miR-485/AIM2通路抑制大脑中动脉闭塞大鼠细胞焦亡的作用机制

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关键词大脑中动脉闭塞;天麻素;细胞焦亡;IncRNA长链非编码核糖核酸母性表达3;miR-485；黑素瘤缺乏因子2

doi：10.12102/j.issn.1672-1349.2025.23.006

Gastrodin InhibitsPyroptosis inMCAORatsbyRegulating the IncRNA Meg3/miR-485/AIM2PathwayVia Exosomes NAN Yinan1，WANG Shaoqing2

1. CorrespondingAuthor WANGShaoqing，E-mail： jwsq2404 @ sina.com

AbstractObjective：Toinvestigatethemechanismbyhichgastrodininhibitspyroptosisinmiddlecerebralarterylusion（MCO）rats throughthelongnon-codingRNA（IncRNA）matemalyexpressdgene3（Meg3）/miR-485/absentinmelanoma2（AIM2）pathwayvia exosomes，therebyprovidinganexperimentalbasisforearlyinterventionwithtradionalChinesemedicineduringtheacutestageof cerebral infarction.Methods：SDatsweredividedintocontrolgroup，shamoperationgroup，modelgroup，andgastrodingroup.emodel group and the gastrodin group were subjected with MCAO surgery.Gastrodin at a dose of 100mg/（kg⋅d） wasadministered intraperitoneally.SD ratsweresacrficdondays13and7.Teischemichemisphereandserumwerecolected.Exosomeswereextractedfromtheseru. Thelevelsof NOD-likereceptorprotein3（NLRP3）andAIM2intheischemichemispheresweredetectedbyWestemblotingand immunohistochemistryThelevelsofNLRP3andAIM2 mRNA，as wellasIncRNA Meg3andmiR-485，intheischemichemispheresof ratsineachgroupweredetectedbyquantiativeeal-timepolymerasechainreaction（qPCR）.Exosomeswereisolatedfromperipheral plasmaandthelevelsofncRNAMeg3andmiR-485weremeasuredbyPCRResults：WestemBlotresultsshowedthatthecontentsof NLRP3and AlM2 intheischemic hemisphere of gastrodin group were lower than those inthe model groupe （P<0.05） .The immunohistochemicalresultsshowedthesametrend.TheqPCRresultsshowedthatthemRNAlevelsofAIM2intheischemic hemisphereofratsintegastrodingroupoestrdand7tdaystercerebralinfarctionwereallowerthantoseinodel group P<0.05 or P<0.01 ）.The IncRNA Meg3 level wasalso lower than that in the model group（ P<0.05 or P<0.01 ），while themiR-485 level was higher than that in the model group P<0.05 or P<0.01 ）.The content of IncRNA Meg3 in serum exosomes of the ginsenoside group was lower than that of themodel grouponthe1st，3rd，and7thdaysaftercerebral infarction P<0.05 or P<0.01 ），whilethecontent of miR-485 was higher than that of the model group（ P<0.01 ）.Conclusion：Gastrodin probably inhibits pyroptosis in MCAO rats by regulating the IncRNA Meg3/miR-485/AIM2 pathwayviaexosomes.

Keywordsmidlecerebralarterylusion;gastrodin;pyoptosis;longnon-codingRNAmateallexpressedgene3;mi-485bsent inmelanoma2

缺血性中风是危害人类健康的重大疾病，炎症是其重要的病理机制1，近年研究发现细胞焦亡也参与调控脑梗死后炎症反应过程[2-3]。（剩余11168字）