基于微生物学的速食食品致病菌检验研究

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摘 要：分析基于微生物学的速食食品致病菌检验检测结果，总结相应的检验方法。采用传统细菌分离培养法和实时荧光定量PCR 法进行检验。对两种检验方法下的致病菌阳性检出情况进行统计，计算出总阳性率。并对两组检验所耗费的时间进行记录，计算出平均用时结果。经统计与组间比较，实时荧光定量PCR 法的样本阳性检出率为11.00%，较传统细菌分离培养法的8.00%明显较高，P

关键词：速食食品;致病菌;检验;实时荧光定量PCR 法;传统细菌分离培养法

中图分类号：TS207 文献标识码：A 文章编号：1001-5922（2022）04-0084-04

Abstract：  To analyze the test results of pathogenic bacteria in fast-food food based on microbiology and summarize the corresponding test methods. Traditional bacterial isolation and culture method and real-time fluorescent quantitative PCR method were used for detection. Count the positive detection of pathogenic bacteria under the two test methods， and calculate the total positive rate. Meanwhile， record the time spent in the two sets of tests， and calculate the average time. According to statistics and comparison between groups， the positive detection rate of real-time fluorescent quantitative PCR method was 11.00%， which was significantly higher than the 8.00% of traditional bacterial isolation and culture method， and P

Key words：  fast food;pathogenic bacteria;inspection;real-time fluorescent quantitative PCR method;traditional bacterial isolation and culture method

目前，食品安全因为与人们的生活和健康息息相关，已经成为全社会广泛关注的热点话题[1]。（剩余6028字）