通过生物信息学分析鉴定肾透明细胞癌中的候选核心基因及其与免疫细胞浸润的关系

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[关键词］肾透明细胞癌;生物信息学分析;核心基因；生存期；免疫浸润

[中图分类号]R737.11[文献标志码]A［文章编号]1671-7783（2025）06-0512-09 DOI： 10.13312/j.issn.1671-7783.y240224

[Abstract]Objective： Screening and identifying of core genes in clear cell renal cell carcinoma （cRCC） based on bioinformatics analysis techniques to search for novel biomarkers for the diagnosis of ccRCC and therapeutic targets.Methods： Diffrentially expressed genes （DEGs） between ccRCC samples and normal kidney samples were obtained from three datasets （including GSE100666，GSE168845，and GSE96574） using the GEO2R tool； DAVID online tool was used to annotate the intersection DEGs of these three datasets with GO and KEGG functions；A protein interaction network of these intersecting DEGs was constructed using the STRING website，and then the core genes through the CytoHubba plugin in Cytoscape software were determined; The expresion of core genes in ccRCC were verified using data from Oncomine and TIMER 2.O databases，the relationship between core genes and the survival of ccRCC patients were further analysed using GEPIA database，and also the relationship between core genes and immune cell infiltration were further analysed using TIMER 2.O database.Results： A total of 189 DEGs were screened，which involve various biological functions and pathways such as tumor growth regulation. Ten DEGs were identified as core genes， including PTPRC，CTLA4，CCR5，CXCL9，GZMB，CD27， CXCL13， IDO1，LCP2，and CASR. The expression analysis results of GEO dataset， Oncomine，and TIMER database allshowed that compared with normal samples，the expression of CASR in ccRCC was significantly reduced，while the expression of the other 9 core genes was significantly increased. Survival analysis showed that CTLA4， CXCL13，and CASR were assciated with overall survival in ccRCC patients，while CXCL13 and CASR were associated with disease-free survival in ccRCC patients. The TIMER 2.O results indicated that upregulation of core gene expression was associated with immune cell infiltration in ccRCC. Conclusion：Ten core genes were identified that may be closely related to the pathogenesis of ccRCC，which may become new diagnostic markers and therapeutic targets for ccRCC.

[Key words]clear cell renal cell carcinoma；bioinformatics analysis； hub gene； survival; immuneinfiltration

肾细胞癌约占成人恶性肿瘤的 5% ，是威胁全球人类健康的恶性肿瘤之一[1]。（剩余13937字）