含SET结构域赖氨酸甲基转移酶5对卵巢癌细胞增殖和迁移的影响

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[中图分类号]R737.31 ［文献标志码］ A [文章编号] 1671-7783（2025）06-0496-08

[Abstract]Objective： To investigate the expression and significance of SET domain containing lysine methyltransferase 5（SETD5）in ovrian cancer tissues and cellines.Methods：The correlation between SETD5 mRNA expression and the prognosis of patients with ovarian cancer was analyzed by Kaplan-Meier survival curves based on public database. Immunohistochemical staining was used to detect the expression level of SETD5 in 88 cases of ovarian cancer tissues and 8 cases of normal ovarian tissues.Western blotting was used to detect the expression levels of SETD5 in ovarian granulosa cells KGN，ovarian endometrioid adenocarcinoma cells A2780，ovarian serous adenocarcinoma cells SKOV3，epithelial ovarian cancer cells Hey A8 and ovarian serous adenocarcinoma cels ES-2. The SKOV3 cell line of ovarian serous adenocarcinoma was selected to construct a stable overexpression cell line of SETD5，and two SETD5-targeting shRNAs were used to construct a stable knockdown celline of ovarian endometrioid adenocarcinoma A2780 cell line. The migration ability of SKOV3 and A2780 cells was detected by

Transwell and wound healing assays，and the proliferation ability of SKOV3 and A278O cells was detected by cell ounting and CCK-8 assay. Results： The Kaplan-Meier survival analysis curves showed that high expression of SETD5 mRNA was assciated with shortened progression-free survival and overall survival in patients with ovarian cancer. The immunohistochemical results of tissue microarray showed that the positive expression rate of SETD5 protein in ovarian cancer tisses was significantly higher than that in normal ovarian tissues（ χ2=7.308 ， P=0.007 ）. The expression level of SETD5 was significantly correlated with the tumor diameter （ P=0.003 ），Tstage （ P=0.017 ），and FIGO stage（ P=0.012 ）of patients with ovarian cancer.The results of in vitro functional experiments showed that overexpression of SETD5 significantly promoted the proliferation and migration ability of SKOV3 cells，while knockdown of SETD5 significantly inhibited the proliferation and migration of A278O cells. Conclusion： SETD5 is highly expressed in human ovarian cancer tissues as well as SKOV3 and A278O cells，and is associated with the prognosis of patients， promotes the proliferation and migration of ovarian cancer cells.

[Key words] SET domain containing 5；prognosis；migration； proliferation； ovarian cancer

卵巢癌发病隐匿，缺乏有效的诊断和监测标志物， 75% 患者在确诊时已是晚期，五年内生存率仅为50%[1] 。（剩余13083字）