硒镁纳米颗粒的制备及其抗菌效果评价

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[中图分类号] R318.08;R587.2 ［文献标志码］A [文章编号] 1671-7783（2025）04-0355-06

DOI： 10.13312/j.issn.1671-7783.y240041

[引用格式].硒镁纳米颗粒的制备及其抗菌效果评价[J].江苏大学学报（医学版），2025，35（4）： 355-360.

Preparation of selenium-magnesium nanoparticles and their antibacterial effect evaluation

MENG Ye， ZHANG Chun，SHEN Haijun （School of Medicine，Jiangsu University，ZhenjiangJiangsu 212O13，China）

[Abstract]Objective：To integrate the two elements of selenium and magnesium into nanoparticles to prepare selenium-magnesium nanoparticles，and explore their antibacterial effect and antibacterial mechanism. Methods： The morphology and particle size distribution of selenium-magnesium nanoparticles were characterized by transmisson electron microscopy and nano-particle size analyzer. The antibacterial effect of selenium-magnesium nanoparticles was evaluated by plate counting method，disk diffsion method，and bacterial live/dead staining method. The content of reactive oxygen species in bacteria after exposure to nanoparticles was detected by DCFH-DA fluorescence probe to explore its potential antibacterial mechanism.Results： Selenium-magnesium nanoparticles are irregular spherical under transmission electron microscopy，and the particle size is mainly concentrated in the range of 5O-70 nm. They exhibit excellent antibacterial properties，especially against Staphylococcus aureus and methicillinresistant Staphylococcus aureus.The potential antibacterial mechanism involves induction of reactive oxygen species production in bacteria. Conclusion： The prepared selenium-magnesium nanoparticles exhibitgood antibacterial properties，ithas been preliminarilydemonstrated that bacterial kiling is achieved by inducing the production of reactive oxygen species within bacterial.

[Key words]selenium； magnesium； nanoparticles；antibacterial; reactive oxygen species； diabetic ulcer

糖尿病的发病率正在逐年攀升，2021年全球糖尿病患者约为5.37亿，预计到2045年将高达7.83亿[1]。（剩余8301字）