甲基转移酶SETD7促进卵巢癌细胞增殖和迁移

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[关键词］卵巢癌;含有SET结构域的赖氨酸甲基转移酶7（SETD7）;增殖;迁移;上皮-间质转化 [中图分类号]R737.31［文献标志码]A [文章编号] 1671-7783（2025）04-0318-08 DOI： 10.13312/j.issn.1671-7783.y240040

[引用格式]，等.甲基转移酶 SETD7促进卵巢癌细胞增殖和迁移[J]．江苏大学学报（医学版），2025，35（4）：318-325.

Methyltransferase SETD7 promotes the proliferation and migration of ovarian cancer cells

ZHANG Ziwei ， HOU Yanan， HUANG Ting， ZHANG Bowen，LI Mingyang， LIN Qiong， SHAO Genbao（School of Medicine，Jiangsu University，Zhenjiang Jiangsu 212O13，China）

[Abstract]Objective： To investigate the expresson of SET domain containing lysine methyltransferase 7（SETD7） in ovarian cancer and its effect on epithelial-mesenchymal transition （EMT），as well as the proliferation and migration of ovarian cancer cels.Methods： The expression level of SETD7 in ovarian cancer microarrays was detected by immunohistochemical staining，and the correlation between SETD7 expression and clinicopathological features and prognosis of ovarian cancer patients was analyzed. Both stable knockdown SETD7 and overexpression SETD7 cell lines were constructed by using lentiviral expression system；cell counting，CCK-8， Transwell and scratch assay were used to evaluate the proliferation and migration ability of ovarian cancer cells，while Western blotting was used to determine the expression levels of EMT-related proteins. Results： Compared with normal ovarian tissues，the expression level of SETD7 protein in human ovarian cancer tissues was significantly up-regulated（ P< 0.01）. High expression of SETD7 was greatly correlated with M stage， FIGO stage and progression-free survival in ovarian cancer patients（ P<0.05 ）. Knockdown of SETD7 significantly inhibited the proliferation and migration of ovarian cancer A278O cells（ P<0.05 ），while overexpression was the opposite in SKOV3 cells. Overexpression of SETD7 up-regulated the expression of N-cadherin and Snail， and down-regulated the expression of E-cadherin，while its knockdown exerted the opposite in A2780 cells. Conclusion： SETD7 is up-regulated in human ovarian cancer tissues and cels，which promotes the migration，proliferation and EMT process of ovarian cancer cells.

[Key words]ovarian cancer; SET domain containing lysine methyltransferase 7（SETD7）；proliferation ; migration；epithelial-mesenchymal transition

卵巢癌是妇科常见的恶性肿瘤之一，大多数患者表现为隐匿性起病， 75% 患者由于无效诊断，发现时已处于晚期[1]。（剩余12208字）