象草PHR家族基因的鉴定及表达分析

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中图分类号：S54 文献标识码：A 文章编号：1007-0435（2025）07-2099-15

doi：10.11733/j.issn.1007-0435.2025.07.006

引用格式：，等.象草PHR家族基因的鉴定及表达分析[J].草地学报，2025，33（7）：2099—2113 FANG Ting，LUO Jia-jia， ZHANG Yu-ge，et al. Identification and Expression Analysis of the PHR GeneFamily in Pennisetum Purpureum[J].Acta Agrestia Sinica，2025，33（7） ：2099-2113

Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum

FANG Tingl#，LUO Jia-jia 1，2#. ， ZHANG Yu-ge1，MAO Yu-ye1，GUO Yu-tong1， DONG Rong-shu²， CAI Ze-ping1* （1.SchooloficalAgiclureandFestryanUvesityo，inrovie28a;oicalCoetic Resources Institute，Chinese AcademyofTropical Agriculture Sciences，KeyLaboratoryofCropGeneResourcesand Germplasm EnhancementinSouthernChina，MinstryofAgricultureandRuralAfairs，Haikou，HainanProvince57o，China）

Abstract：The phosphate starvation response （PHR） factor plays a pivotal role in regulating phosphorus homeo stasis in plants，yet the PHRs in Pennisetum purpureum （elephant grass）remain unreported. This study analyzed the efects of low phosphorus （P）stress on the growth of elephant grass，identified the members of PHR family in elephant grass，and examined their transcription expression levels.Furthermore，the two key genes P/PHR/13 were cloned，and their expression patterns and subcellular localization were analyzed. The results demonstrated that low P stress significantly inhibited the growth of elephant grassA total of 42 PpPHRs were identified from the elephant grassgenome，with colliear gene pairs originating from whole-genome duplication or segmental duplication events. Transcriptome showed that 1 5PρPHRs were significantly upregulated by low P stress in elephant grass leaves（and/or roots），with P/PHR/13 exhibiting relatively high expression levels. Additionally， tissue-specific expression analysis revealed that P/PHR/13 had the highest expression levels in roots and stems，respectively. Quantitative PCR confirmed that low P treatment enhanced the expression of

P/PHR/1/3 in both roots and leaves. Subcellular localization indicated that both P/PPHRδ andPpPHR13pro teins were localized in the nucleus. This study provides candidate gene resources for elucidating the mechanisms underlying elephant grass's response to phosphorus deficiency.

Key Words：Pennisetum purpureum;Low phosphorus stress ;Phosphate starvation response factor;Gene expres sion;Subcellular localization

磷（Phosphorus，P）是植物生长所必需的大量营养元素之一，是生物大分子（如核酸、磷脂和三磷酸腺苷ATP等）的基本结构组分，主要通过磷酸化和去磷酸化作用参与光合作用、能量代谢和细胞信号转导等多种重要生物过程[1]。（剩余23283字）