神经性勃起功能障碍大鼠模型的建立与评价

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中图分类号：R285.5文献标志码：A文章编号：1003-9783（2025）06-0950-08

doi：10.19378/j.issn.1003-9783.2025.06.012

Establishment and Evaluation A Rat Model for Neurogenic Erectile Dysfunction

LIANG Lang1 ，LU Xiaomeng²，LI Hongliang1，LIU Jiayel，YE Shenglian1，CAO Hongying1（1. 51o6 Guangdong，China；2.Guangdong Xinxing Junior College the Medicine，Yunfu 5274Oo Guangdong，China）

Abstract：Objective To establish a rat model neurogenic erectile dysfunction （ED）and evaluate it comprehensively，while exploring the potential pathogenesis the model.MethodsSDrats were randomlydivided into a sham-operated group and aneurogenic ED group.The neurogenic ED group underwent bilateral cavernous nerve crush surgery to establish the model.Three weeks post-surgery，therats’erectilefunction was assessed using mating behavior tests andintracavernous pressre （ICP）measurements.Masson staining was usedto evaluate the level fibrosis in the corpus cavernosum，and invitro tension experiments were conducted toassess the relaxation function cavernous smooth muscle.Immunluorescence was used to measure nitric oxide（NO）release in the corpus cavernosum，and enzyme-linked immunosorbent assy（ELISA）was used to determine the cyclic guanosine monophosphate （cGMP） content inthe corpuscavernosum，aiming to explorethe potential pathogenesis the model.Results Compared with the sham-operated group，the neurogenic ED group showed a significant reduction in the number captures and intromissions（ P<0.05 ），prolonged intromission latency（ P<0.05 ），decreased intracavernous pressure（ P<

0.001），increased fibrosis in the corpus cavernosum （ P<0.05 ），enhanced phenylephrine （PE）-induced contraction cavernous smooth muscle（ P<0.05， ，and reduced electrical field stimulation（EFS）-induced relaxation cavernous smooth muscle （P<0.01，P<0.001， ）.Additionally，the expression levels NO and cGMP in the corpus cavernosum were significantly reduced （P<0.01，P<0.001） . Conclusion This study successfully established a rat model neurogenic ED using bilateral cavernous nerve crush surgery.The pathogenesis may be related to the downregulation the NO-cGMP pathway.This model provides a reliableanimal model for further research on the efficacy evaluation traditional medicine and the exploration pharmacological mechanisms.

Keywords：cavernous nerve；neurogenic erectile dysfunction；animal model；cavernous smooth muscle； nitric oxide；rats

勃起功能障碍（ErectileDysfunction，ED）是一种常见的男性性功能障碍，其发病原因复杂，包括神经性因素、血管性因素、内分泌因素等。（剩余13039字）