基于氨基酸类成分定量结合化学计量学评价不同产地的地龙质量

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中图分类号：R283 文献标志码：A 文章编号：1003-9783（2025）05-0791-07

doi：10.19378/j.issn.1003-9783.2025.05.014

QualityEvaluation of Pheretima from Different Origins Based on Quantitative Analysis of Aminc Acid Components Combined with Chemometrics

CHEN Xueting1，ZHANG Ting²，WANG Sifeng²，ZHANG Jianjun’，MAI Bingming¹，LI Shaolin（1.Guangdong SecondTraditional ChineseMedicine Hospital/Guangdong Research Instituteof Traditional Chinese Medicine Manufacturing Technology，Guangdong Provincial Key Laboratoryof Researchand Developmentin Traditional Chinese Medicin，Guangzhou 510095Guangdong，China；2.TheFifth Clinical Medical School of Guangzhou Universityof Chinese Medicine，Guangzhou 510095 Guangdong，China；3.Guangdong Food and Drug Vocational Collge， Guangzhou 510520 Guangdong，China）

Abstract：Objective To establishamethod fordetermining thecontentofamino acid components in Pheretima and to evaluatethequalityof pheretima from differentorigins.Methods Thecontents of amino acid components in24 batches of Pheretima from diferent origins were determinedby high-performance liquid chromatography（HPLC）.Principal component analysis（PCA）and partial least squares-discriminant analysis（PLS-DA）were used to evaluate the quality of Pheretima.Results Aspartic acid，glutamic acid，serine，histidine，glycine，arginine，proline，alanine， valine，methionine，isoleucine，leucine，tryptophan，phenylalanine，lysine，and tyrosine showed good linear relationships within their respective ranges（ r>0.999 ）. The average recovery rates ranged from 98.73% to 102.56% ， with RSD values ranging from 1.93% to 3.18% ，meeting the requirements of the Chinese Pharmacopoeia for content determination.PCA extracted three principal components with a cumulative variance contribution rate of 82.493% ： Pheretimasamples from the Guangdong and Guangxi regions had higher comprehensive scores and ranked at the top. PLS-DA indicated that proline，methionine，histidine，alanine，phenylalanine，and tryptophan were the main differential markers afecting thequalityof Pheretima.Conclusion The evaluation method basedon quantitative analysis of amino acid componentscombined with chemometrics can beused to study thequality diferences of Pheretima from different origins.

Keywords：Pheretima；amino acids；origin；quality evaluation

地龙药用历史悠久，始载于《神农本草经》，具有清热定惊、通络、平喘、利尿的功效-2]，广泛用于治疗高热神昏、惊痫抽搐、关节痹痛、肢体麻木、半身不遂、肺热喘咳、水肿尿少等病症3-5。（剩余6739字）