miR-193a-5p在脂多糖诱导的奶牛乳腺上皮细胞炎性反应中的作用

打开文本图片集

中图分类号：S823.8 文献标志码：A 文章编号：1001-411X（2025）06-0872-11

Abstract： 【Objective】This study aims to investigate the role of miR-193a-5p in bovine mastitis by constructing an inflammatory response model of lipopolysaccharide （LPS）-induced bovine mammary epithelial cels line （MAC-T）. 【Method】 The miR-193a-5p expression level in LPS-induced MAC-T cels inflammatory response model was detected. By overexpressing miR-193a-5p in MAC-T cells， qPCR， CCK-8 and EdU assays were used to examine expressionchanges of inflammatory factors，proliferation- and apoptosis-related genes，as well as cell viability and proliferation.Bioinformatics methods were employed to predictcandidate target genes of miR-93a-5p，and GO and KEGG analyses were conducted to reveal its potential mechanisms of action. 【Result】 The miR-193a-5p expression levels were significantly downregulated at 3， 6 and 12h after LPS induction in MAC-T cells （P<0.01 ）. Overexpression of miR-193a-5p promoted the expressions of inflammatory cell factors [L-lβ and IL-8） and apoptosis-related gene （ （CASP9） ，andinhibitedthe expressionof proliferationrelated genes （BCL2 and PCNA， ）. Furthermore，miR-193a-5p overexpression suppressed cell viability and proliferation. A total of 44 candidate target genes of miR-193a-5p were predicted. These genes were enriched in pathways including ribosome biogenesis in eukaryotes，adherens junction， NF- σ⋅κB signaling pathway， Wnt signaling pathway，and Alzheimer disease，and were primarily involved inbiological processes such as the regulation of cell differentiation and regulation of cytokine production. 【Conclusion】 The miR-193a-5p expression is downregulated in LPS-induced MAC-T cells. Overexpression of miR-193a-5p potentially promotes inflammatory responses， inhibits proliferation， and induces apoptosis through the NF- ⋅κB and Wnt signaling pathways， suggesting its regulatory role in the bovine mastitis pathogenesis.

Key words： Dairy cow; LPS; Mammary epithelial cell; miR-193a-5p; Mastitis; Inflammatory response

在奶牛养殖业中，乳腺炎具有极高的发病率，严重影响奶牛的生产性能及乳品质量[1]。（剩余16909字）