基于PCR-CRISPR/Cas12a技术的肠炎沙门菌快速检测方法

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中图分类号：S852 文献标志码：A 文章编号：1001-411X（2025）03-0311-08

A fast method for detecting Salmonella enteritidis based on PCR-CRISPR/Cas12a

YANG Tianmut， WANG Yiheng†， XIONG Wenguang， GUO Jianying （Guangdong ProvincialKeyLaboratoryofVeterinaryPharmaceuticsDevelopmentandSafetyEvaluation/NationalLaboratoryof Safety Evaluation （Environmental Assessment） of Veterinary Drugs/National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria/College of Veterinary Medicine，South China Agricultural University， Guangzhou 510642， China）

Abstract： 【Objective】To realize early detection， preventionand control of Salmonela enteritidis，a detection method was established by combining CRISPR（Clustered regularly interspaced short palindromic repeat） technology with polymerase chain reaction （PCR）.【Method】 PCR primers were screened based on the conserved sdfI gene sequence. The sensitivity test was conducted using S. enteritidis genomes at diferent concentrations，whilethe specificitytests wereconducted using thegenomes ofEscherichiacoli，Staphylococcus aureus，Pasteurellamultocida，amonellacholeraesuisandEnterococcusfaecalis.Subsequently，thedeveloped method was applied for the detection of S ， enteritidis in small intestinal samples. 【Result】 This method was capable of identifying S. enteritidis in small intestinal samples， with a detection limit of ，showing excellent specificity and no cross-reactivity with E coli，S.aureus，P.multocida，S.choleraesuisand E faecalis 【Conclusion】 This study develops a compact diagnostic method for the early detection of S . enteritidis， showing excellnt sensitivity and specificity. This innovation presents a fresh perspective for the swift identificationofS.enteritidis.

Key words： Salmonella enteritidis; sdfI gene; PCR; CRISPR/Cas12a system

禽肉和禽蛋是人类优质蛋白质的重要来源，禽生长迅速、出栏较快，在中国居民膳食的动物性食物中占较大比例。（剩余11923字）