DNA分子鉴定和理化分析相结合对灵芝质量评价的研究与应用

打开文本图片集

关键词：灵芝；DNA分子鉴定；理化分析；质量评价

中图分类号：R282.5 文献标志码：A 文章编号：1007-3213（2025）04-0988-04

DOI：10.13359/j.cnki.gzxbtcm.2025.04.027

Research and Application of Quality Evaluation of Ganoderma lucidum Based on the Combination of DNA Molecular Identification and Physicochemical Analysis

ZHANG Qingying，DONG Yanyan （Liaocheng Vocational and Technical College，Liaocheng 252Ooo Shandong，China）

Abstract：Objective The quality evaluation of 2O batchesof Ganoderma wascariedout bythe combinationof DNA identification and physicochemical analysis.Methods The 20 batches of Ganoderma were identified by DNA extraction，polymerase chain reaction （PCR）amplification，purification，sequencing，and inputing the Chinese medicines into DNA barcode identification system.Then the content determination of marker components was carried out.Results Ganoderma of No.1，2，3，4，5，6，9，10，11，12，13，14，15，16，18 and 20 belonged to Ganoderma lucidum（Leyssex Fr.）Karst.in the Pharmacopoeia，while No.7，8，17and 19 did notbelong to Ganoderma lucidum （LeyssexFr.）Karst.in thePharmacopoeia.Contentdetermination was conducted on16 batches of Ganoderma lucidum （Leyssex Fr.） Karst.， in which 15 batches were up to the standards of Pharmacopoeia，the decreasing sequence of contents was as follws：No.2，1，5，10，18，3，4，16，15， 11，13，14，6，9，and 12，and No.2O was failed to meet the standards，with a content of 0.38% . Conclusion The combination of DNA molecular identificationand physicochemical analysis can beused for rapid quality evaluation of the authenticity and superiority of Ganoderma.

Keywords： Ganoderma；DNA molecular identification；physicochemical analysis；quality evaluation

灵芝是利用最早和文化最厚重的食药用菌之一，其市场需求日益增长，已知灵芝种类有88种2、质量参差不齐，严重制约了其临床应用的广泛性和有效性。（剩余4471字）