红景天苷通过下调HMGB1、自噬抑制肝癌细胞增殖和侵袭

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关键词：红景天苷；肝癌；HMGB1；自噬；增殖；侵袭；H22细胞

中图分类号：R285.5 文献标志码：A 文章编号：1007-3213（2025）04-0982-06

DOI：10.13359/j.cnki.gzxbtcm.2025.04.026

Salidroside Inhibits Proliferation and Invasion of Hepatocellular Carcinoma Cells Through Down-regulating HMGB1 and Autophagy

YANGLimin'，LI Shanlin²，WANGJin1 （1.Dept.of Gastroenterology，TheFirstAfiliated Hospitalof Zhengzhou University，Zhengzhou450052Henan，China; 2.Dept.of Gastroenterology，Zhoukou Central Hospital，Zhoukou 466O99 Henan， China）

Abstract：Objective To observe the mechanism of anti-hepatocellular carcinoma of salidroside.Methods H22 hepatocellular carcinoma cells were divided into control group（only fresh culture medium was replaced）and salidroside low ，medium- and high dose groups，and the corresponding intervention was given in each group for 48 hours. Cellular proliferation activity was detected by cell counting kit（CCK） -8 ，the number of invasive cells was detected by Transwell assay，the number of autophagosomes was observed by using dansylcadaverine （MDC）staining method，the mRNA and protein expression levels of high mobility group protein 1（HMGB1）and autophagy-related genes yeast Atg6 homolog（Beclin1），microtubule-associated protein1A/1B-light chain 3 （LC3 II/I）and sequestosome 62（p62） were detected accordingly byreal-time quantitative polymerasechain reaction （qRT-PCR）and Western Blot，respectively.Results Compared with the control group，the cellular proliferation activity was decreased，the numberof invading celsand autophagosomes were reduced insalidrosidelow-，mediumand high-dose groups，mRNA and protein expresion level and of p62 were increased，and mRNA and protein expressionlevels of HMGB1，LC3-II/I and Beclin1 weredecreased，the diferences being statistically significant （ P<0.05 ），showing a dose-dependent manner. Conclusion Salidroside may inhibit the proliferation and invasion of hepatocellular carcinoma cels by down-regulating the expression of HMGB1 and autophagy level.

Keywords：salidroside；hepatocelular carcinoma；HMGB1；autophagy；proliferation； invasion；H22 cells

肝癌具有隐匿性、转移早、进展快以及侵袭性强等发病特点，发病率呈逐年上升趋势，目前，手术切除、肝移植和化疗是临床治疗肝癌的主要手段，但是，仍存在耐药性、预后不良以及5年生存率低等情况，因此，寻求新的有效治疗肝癌的方法具有重要意义。（剩余8595字）