CRISPR-Cas介导基因编辑技术的发展趋势及研究进展

摘要：CRISPR-Cas系统为细菌与古生菌的一种免疫防御机制。基于该系统的基因编辑技术在基因工程领域得到广泛应用，极大地加快了该领域研究的进步。本文从CRISPR-Cas系统介导的基因组编辑技术的发展趋势和最新应用及现状进行综述。

关键词：CRISPR-Cas系统 ; Cas9 ; 基因组编辑技术

中图分类号：Q78 文献标识码：A 文章编号：2096-3718.2022.06.0.06

Development tendency and research progress of CRISPR-CAS-based Genome engineering

YANG Yongchang， CHANG Shuai， ZHAO Xinyu

（Department of Clinical Laboratory， Seventh Medical Center of Chinese PLA General Hospital， Beijing 100010， China ）

Abstract： CRISPR-Cas system is used to resist exogenous viruses or plasmids in bacteria and archaea Acquired immune system of DNA invasion. The gene editing technology based on this system has brought a breakthrough revolution to the regulation and application of targeted genome modification. In this paper， we review the development and application of CRISPR-Cas system mediated genome editing technology， and provide reference for related researchers.

Keywords： CRISPR-CAS system; Cas9; Genome engineering

基因组编辑技术的迅猛发展引起科学界的广泛重视。（剩余16433字）