聚苯乙烯纳米塑料和Cu2+对施氏假单胞菌的复合胁迫研究
打开文本图片集
中图分类号：X703 文献标志码：A 文章编号：1000-582X（2025）12-020-13
Abstract： The coexistence of polystyrene nanoplastics （PS-NPs） and Cu2+ in wastewater can induce compound stress effects on microorganisms. This study investigated the effects of PS-NPs and Cu2+ on the growth， nitrogen removal，and biochemicalcharacteristicsoftheaerobicdenitrifying bacteriumPseudomonasstutzeri，andfurther elucidated the stress mechanism through transcriptomics analysis.The results showed that under exposure to 50mg/L PS-NPs， bacteria growth activity increased by 25.3% compared to the control， accompanied by enhanced nitrogenremoval and significant upregulation of genes related to ribosome function，the tricarboxylic acid（TCA） cycle，and ABC transport pathways. In contrast， under 10mg/L （204号 Cu2+ stress，bacterial growth activity decreased by83.2% compared to the control，with severe inhibition，cell membrane damage，and marked downregulation of ribosomal，nitrogen metabolism，and ABC transport pathways. Genes related to extracellular polymeric substances （EPS） secretion and cell membrane synthesis were significantly upregulated. Under combined stress， Cu2+ exerted the dominantinhibitory effect;however，PS-NPs promotedEPSsecretion，which，along with PS-NPs themselves， adsorbed part of the Cu2+ ions and mitigated their toxicity.
Keywords： aerobic denitrifying bacteria; polystyrene nanoplastics; copper ion; combined stres;transcriptomics
生物脱氮是废水处理中不可或缺的环节，好氧反硝化菌在生物脱氮中起着重要作用。（剩余19293字）
