IRGM在结直肠癌中的表达及其与患者临床病理特征和预后的关系

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[中图分类号]R375.34 [文献标志码］A

Expression of immune-related guanosine triphosphatase M in colorectal cancer and its association with clinicopathological features and prognosisLI Na，RAN Wenwen ， ZHAO Han， XING Xiaoming（Department of Pathology，School of Basic Medicine，Qingdao University，Qingdao 266O71，China）

[ABSTRACT]ObjectiveTo investigate theexpressioncharacteristicsof immune-related guanosine triphosphatase M（IRGM）incolorectalcancer（CRC）tisueanditsasociationwithclinicopathologicalfeaturesand prognosis.MethodsFreshtumor tissuesamplesandthecoresponding paracancerous normal tissuesamples werecollctedfrom16patientswhounderwentradical resectionfor CRCin The Afiliated Hospitalof Qingdao University，andRT-qPCR was used to measure themRNA expressionlevel of IRGM.Postoperativeparafi-embedded tumor tisue samples and thecorresponding paracancerous normal tisue samples were colectedfrom143CRCpatientswhoweretreatedinTheAfliatedHospitalofQingdaoUniversity，andimmunohistochemicalstaining wasused tomeasuretheproteinexpressonlevelofIRGM;theassociationoftheprotein expresionlevelofIRGMwithclinicopathological featuresandprognosis wasanalyzed.ResultsThemRNAexpresionlevelof IRGM in paracancerous normal tissue was significantly higher than that in CRC tumor tissue （ ）. The protein expression level of IRGM in paracancerous normal tissue was also significantly higher than that in CRC tumor tissue ⟨χ2=23.738，P<0.05⟩ . The low expression of IRGM in CRC tumor tissue was significantly associated with lymph node metastasis and vascular tumor thrombus （ χ2=9.827，7.690 ， （20 P<0.05 ）.The survival analysis showed thatthe expression level of IRGMhad no significantcorrelation with overallsurvival rate or disease-free survival rate in CRC patients （ ΔP>0.05 ）.ConclusionThe expression level of IRGM is significantly downregulatedin CRCtissue；thelowexpressonof IRGMis closelyasociated with lymphnode metastasisandvasculartumorthrombus， whileitisnotsignificantlyassociatedwithteoverallsurvivalrateanddiseasefreesurvivalrateofptients，sugestingthat IGM may become a potential target for the treatment of CRC.

[KEYWORDs]Colorectal neoplasms; GTP-binding proteins;Lymphatic metastasis；Neoplasm invasiveness; Immunohisto chemistry;Prognosis;Pathological conditions， signs and symptoms

结直肠癌（colorectalcancer，CRC）作为全球高发的恶性肿瘤，其早期诊断和治疗策略虽不断进步，但转移性病变的出现仍是导致患者治疗失败和死亡的主要原因[1-2]。（剩余8379字）