脓毒症相关长链非编码RNA筛选及竞争性内源RNA网络构建

打开文本图片集

[中图分类号]R631 [文献标志码]A [DOI] 10.19767/j.cnki.32-1412.2025.05.002

Screening of Sepsis-Associated Circular RNAs and Construction of Competing Endogenous RNA Networks

GUANYuwen，LIANGGuiwen，HUANG Zhongwei，QI Lei， JIANGHaiyan （Departmentof EmergencyMedicine，Afliated Hospital of Nantong University，Jiangsu 226001）

[Abstract]Objective：To explore the regulatoryroleof non-coding RNAs（ncRNAs），particularlylong non-coding RNAs （lncRNAs），inthepathological progressionofsepsis，toidentifynvelbiomarkersforarlydiagnosis，andtoiscover potential therapeutic targets for intervening in immune imbalance.Methods：Peripheral blood samples frompatients with sepsisand healthy individualswerecollected.Wesystematicallyanalyzed the specific expression profilesof lncRNAs in sepsis through ceRNA microarray technology. We investigatedtheregulatory mechanisms oflncRNAs insepsis byintegating theinteractionnetworksof microRNAs（miRNAs）and circularRNAs（circRNAs）.Results：The studyrevealed that IncRNAsexhibitedsignificant diferential expresion insepsis，including248up-regulatedand26down-regulated lncRNAs withdiferential expression.CombiningGOenrichmentanalysisandKEGGpathwayclasificationanalysis，theesults indicatedthat lncRNAsmightparticipate intheregulationof inflammatorystormsand immunosuppressonthrough the ceRNA mechanism.Certain IncRNAs were closely asociated with immune imbalance in sepsis，demonstrating potential as biomarkers or therapeutic targets.Conclusion：lncRNAsplayacriticalrole inthepathological progresionofsepsis.Their specific expresionprofilesprovidenovelcandidate biomarkers forearlydiagnosisof sepsisandoferpotentialtargets for therapeutic strategies aimed at modulating immune dysregulation.

[Keywords] sepsis; non-coding RNA;biomarker

脓毒症（Sepsis）是由于宿主对感染的反应失调，引起危及生命的器官功能障碍I。（剩余10794字）