UFLC-MS/MS法同时测定加味西黄丸中4个胆汁酸成分的含量

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关键词：加味西黄丸；胆汁酸；含量测定；UFLC-MS/MS

中图分类号：R284.1 文献标志码：A 文章编号：1003-9783（2025）11-1949-06

doi：10.19378/j.issn.1003-9783.2025.11.013

Simultaneous Determination of Four Bile Acid Components in Modified Xihuang Pils by UFLCMS/MS

YANG Yan1，2 ，WANG Xing1，²，LI Yuhua³，YUWenjie³，LIU Yang1，²，LI Lele'，GUAN Jiao’，YU Chunyu²，ZHU Heyun'（1.Schoolof Pharmacy，Jilin MedicalUniversity，Jilin132013 Jilin，China；2.ScholofPharmacy，Yanbian University，Yanji 133002 Jilin，China；3.Jilin Jibang Pharmaceutical Co.，Ltd.，Shulan13260O Jilin，China）

Abstract：Objective To establish an ultra-fast liquid chromatography-tandem massspectrometry（UFLC-MS/MS） method for the simultaneous determinationof four bileacid components（glycocholicacid，taurocholicacid，cholic acid，and taurochenodeoxycholicacid）inModified Xihuang Pills.MethodsChromatographic separation was performed on a Shimadzu Shim-Pack XR-ODS column（ 75mm×3.0mm ， 2.2μm ）using a mobile phase consisting of 0.1% formic acid aqueous solution（A）and acetonitrile（B）with gradient elution at a flow rate of 0.5mL⋅min-1 and column temperature of 35°C . An electrospray ionization source in negative ion mode with multiple reaction monitoring （MRM）was used forquantitative analysis of bile acid components in Modified Xihuang Pills.Results Glycocholic acid， taurocholicacid，cholicacid，and taurochenodeoxycholic acid showed good linear relationships within theirrespective concentration ranges （ r>0.9989 ）. The average recoveries ranged from 98.80% to 99.62% withR sD⩽1.52% . Insix batchesof Modified Xihuang Pils，the contents of glycocholic acid，taurocholic acid，cholic acid，and taurochenodeoxycholic acid were 0.064 7-0.076 2，0.083 7-0.096 6，0.605 0-0.681 8，and 0.0035-0.0041mg⋅g-1 ， respectively.Conclusion The established method is simple，rapid，and highly sensitive，providing a scientific basis forqualitycontrolofModified XihuangPills. Keywords：Modified Xihuang Pills；bile acids；content determination；UFLC-MS/MS

加味西黄丸是由体外培育牛黄、蟾酥（制）、人工麝香、乳香（制）和没药（制）5味药材组成，具有解毒散痈、祛肿止痛的功效，对治疗热毒血癡导致的痈疽疮疡、瘰疬、牙龈肿痛及各种脓肿等炎症疾病有显著疗效。（剩余8139字）