铁皮石斛抗逆、促生长菌的筛选及其特性分析

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中图分类号：S567.23 文献标志码：A 文章编号：1673-3851（2025）11-0836-08

引文格式：．铁皮石斛抗逆、促生长菌的筛选及其特性分析[J」.浙江理工大学学报（自然科学），2025，53（6）：836-843.

Reference Format：LIU Shimin，GAO Yuanyuan，HU Xiufang. Screening and characteristic analysis of stress-resistant and growth-promotingbacteria inDendrobiumoffcinaleJ].Jouralof Zhejiang Sci-TechUniversity，2025，53（6）86-843.

Screening and characteristic analysis of stress-resistant and growth-promoting bacteria in Dendrobium officinale

LIU Shimin1 ，GAO Yuanyuan² ，HU Xiufang1 （1.College of Life Sciences and Medicine， Zhejiang Sci-Tech University， Hangzhou 31oo18，China; 2. Jining Academy of Agricultural Sciences， Jining 272O3l，China）

Abstract： To screen microbial strains with stress resistance and growth-promoting potential， endophytic bacteria were isolated from the roots，stems，and leaves of Dendrobium oficinale collected from four geographical regions in China. These strains were subjected to simulated high-temperature， drought，acidic，oligotrophic，and oxidative stress conditions for screening. Their growth-promoting capabilities were evaluated by measuring indole-3-acetic acid （IAA） synthesis， phosphate solubilization， and siderophore production. Results revealed that the niche distribution of 172 isolated strains from the four regions exhibited tissue specificity，with Bacillus species colonizing 51.16% of the root. Among 21 high-temperature and drought-resistant strains， Bacillus accounted for 76.19% . Six strains with superior stress tolerance were identified under acidic，oligotrophic，and oxidative stress conditions，among which strain JHGO3 demonstrated optimal acid and oligotrophic resistance. Strain JHGO3 showed significant enrichment and possessed the abilities of phosphate solubilization，siderophore production，and indole-3- acetic acid production.By screening microbial strains demonstrating thermotolerance-enhancing capabilities，the research provides theoretical basis and strain repositories for the development of planttailored bioinoculants against thermal stress.

Key Words： Dendrobium officinale； screening bacteria； ecological niche distribution； hightemperature and drought； growth promotion

0 引言

全球气候变化加剧极端高温频发，而高温会破坏植物膜系统稳定性、抑制酶活性及干扰次生代谢通路（如黄酮类化合物合成受阻），导致药用植物活性成分流失与产量下降[1]。（剩余9390字）