VEGFR2基因靶向RNA纳米颗粒抑制剂的研究
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中图分类号：Q781 文献标志码：A 文章编号：1673-3851（2025）11-0827-09引文格式：肖楚琼，郭玉琴，金伟波，等.VEGFR2 基因靶向RNA纳米颗粒抑制剂的研究[J]．浙江理工大学学报（自然科学），2025，53（6）：827-835.Reference Format： XIAO Chuqiong，GUO Yuqin，JIN Weibo，et al.An investigation into VEGFR2-targeted RNA-basednanoparticle inhibitors[J]. Journal of Zhejiang Sci-Tech University，2O25，53（6） ：827-835.
An investigation into VEGFR2-targeted RNA-based nanoparticle inhibitors
XIAO Chuqionga ，GUO Yuqinb，JIN Weiboa ，LU Zhengbinga （a. College of Life Sciences and Medicine；b. Library， Zhejiang Sci-Tech University， Hangzhou 310018， China）
Abstract： To develop a tumor-suppressive agent targeting the vascular endothelial growth factor （VEGF） and its receptor VEGFR2 signaling pathway，multiple efficient smallinterfering RNA （siRNA） sequences were selected through bioinformatic analysis and assembled into a circular KD-VEGFR2-RNA nanoparticle capable of self-assembly. The nanoparticle was efficiently produced using an Escherichia coli expression system，and its structure and anti-angiogenic activity were characterized both in vitro and in vivo. Results showed the KD -VEGFR2-RNA nanoparticle had a stable structure with diameters ranging from 100nm to 200nm . At a concentration of 25.0ng/μL ，the nanoparticle exhibited excellent biosafety with HUVEC cels， significantly inhibiting HUVEC migration and tube formation capabilities， with corresponding inhibition rates of 57.57% and 64.77% . The nanoparticle could reduce both VEGFR2 mRNA levels and protein expression，demonstrating anti-angiogenic activity in vivo. The KD -VEGFR2- RNA nanoparticle prepared in this study exerts anti-angiogenic activity by synergistically suppressing the VEGFR2 signaling pathway and offers a promising strategy for targeted cancer therapy.
Key Words： VEGFR2； RNA interference; angiogenesis; nanoparticles; tumor inhibitor
0引言
血管内皮生长因子受体（Vascularendothelialgrowth factorreceptor，VEGFR）是表达在内皮细胞膜上的一类跨膜蛋白，血管内皮生长因子（Vascular endothelial growth factor，VEGF）作为VEGFR的配体，可以与其结合，进而参与调控人体血管和淋巴管的生成[1-2]。（剩余12290字）
