高通量环状RNA测序揭示hsacirc0001900在肾母细胞瘤中特异性高表达且与不良预后相关

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Abstract： ObjectiveToexploretheexpressionprofileofcircularRNAs （circRNAs）andtheir potentialroles inprognosisand progression of Wilms'tumor （WT）.MethodsFour pairs of WTandadjacent tissues were colectedfor high-throughput circRNAsequencingtoidentifythediferentiallyexpressedcircularRNAs.RT-qPCRwasusedtoverifytheexpressonlevels ofthetop6candidatecircRNAsintheclinicalsamples.hsa_circ0o900wasselectedforanalysisof itscorelationwith clinicopathologicalfeaturesandprognosisin34patients withWT.SangersequencingandRNaseRdigestionexperimentswere usedtoverifythecyclingsiteandstructural stabilityofhsa_circ_0o190molecule.ResultsAtotalof23978circularRNA moleculeswereidentifiedin WTtisuesbyhigh-throughputcircularRNA sequencing，andamong them 614were diferentiallexpressedinWT.hsa_circ0o9oshowedthehighestexpressionlevelamongthediferentiallyexpressed circRNAs，whichwasconsistentwiththefindingsinclinicaltumorsamplesandthesequencingresults.Corelationanalysis showedthathsacirco19oexpressionlevelwaspositivelycorrelatedwithWvolume，andthechildrenwithhighhsacic 0001900 expresion hadalowered recurrence-free survivalrate.The resultsof Sanger sequencing verifiedthe cicular splice sitesequenceof themolecule，andRnaseR digestionassayconfirmed itsstablecovalentstructure.Conclusion This study presentsacomprehensiveexpressnprofileofiularRNAsinWandtheexpreiolevelofhsa_cio9isatedto thesize ofWTandthepatients'prognosis，suggesting itspossibleroleasakeydriving gene inWTprogresion.

Keywords：nephroblastoma;Wilmstumor;circularRNA;hsa_circ_0001900

环状RNA是一类新发现的非编码RNA，主要通过miRNA“海绵"机制[发挥转录后基因表达调节作用[2.3]。（剩余16064字）