基于聚合酶链式反应检测糖基化修饰水平

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中图分类号：Q53 DOI：10.16152/j. cnki. xdxbzr. 2025-06-014

Detection of glycosylation modification levels based on polymerase chain reaction

TAN Zengqi¹，ZHANG Huina²，CAO Lin

（1.Shaanxi Key Laboratory of Biotechnology and Biochemical Engineering，School of Medicine， Northwest University，Xi'an 710069，China； 2.Key Laboratory of Resource Biology and Biotechnology in Western China，Ministry of Education/ College of Life Science，Northwest University，Xi'an 71OO69，China）

AbstractGlycosylation is an essential post-translational modification. Over 50% of proteins in the human body undergo glycosylation，which can influence their biological functions and participate in various biological processes.Abnormal glycosylation occurs in a range of physiological and pathological processes，such as cancer，inflammation，or metabolic diseases，making the detection of glycosylation essential.Classical methods for detecting glycosylation，like flow cytometry and mass spectrometry，often struggle with trace-level glycosylation signals.This study reports a DNA amplification-based method for glycan detection. Lectins that recognize and bind certain glycosylations are labeled with single-stranded DNA （ssDNA）.The specific binding between lectins and glycans convert the "glycan signal" into a "nucleic acid signal". Using the ssDNA as a template for PCR amplification enables the detection of trace glycosylation signals，offering a new perspective for glycobiology research and the discovery of clinical glycomarker.

Keywordsglycosylation； lectin;PCR；ssDNA

蛋白质糖基化修饰作为一种常见的翻译后修饰[1]，其过程是在糖基转移酶的作用下，将糖类分子（寡糖或多糖）连接到蛋白质的特定氨基酸残基上，形成糖蛋白[2-3]。（剩余15664字）