丹参多酚酸盐通过MAVS-NLRP3-GSDMD信号通路介导细胞焦亡途径对抗心肌毒性的机制研究

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关键词细胞焦亡；心肌毒性；丹参多酚酸盐；线粒体抗病毒信号蛋白;NOD样受体热蛋白结构域相关蛋白3；半胱氨酸天冬氨酸蛋白酶1；实验研究

doi：10.12102/j.issn.1672-1349.2025.20.005

SalvianolicAcidSaltsMediatetheCellPyroptosisPatwaythroughtheMAVS-NLRP3-GSDMDSignalingPathwaytoCounterac Myocardial Toxicity

LIUJiang'，LI Jian²

QingdaoUvesityinda66o;AdHspialfQiaoUivesityinda6a CorrespondingAuthorLIJian，E-mail： leerabbity @ 126.com

AbstractObjective：Tostudtheprotectiveefectofsavianolicacidsaltsondoxorubicinducedmyocardatoxicityandtheectson themitocondrialantivialsignalingprotein（MAVS）-NOD-likereceptorpyrindomain-containingprotein3（NRP3）gasderminD（GDMD） signalingpathwayincellpyroptosis.Methods：Sixtyratswererandomlydividedintothecontrolgroup，modelgroup，low-dosegroup， medium-dosegroupandigdsegroup.Amyocardialinjuymodelwasstablishdbytraperitonealinectioofdoxoubicinite low-，medium-，and high-dose groups were intervened by intraperitoneal injectionof 10，20，and 40mg/|kg| salvianolic acid salts， respectively.After4weeks，ardiacfunctioninicatorsweredetectedbytrasoundaematoxyliosinsainingaspfodfor pathologicaltiseoetiondtxprsioeesofntioidaticatorllstadpeineesofV NLRP3-GSDMDsignalingpathwayinmyocardialtisse，weremeasuredResults：Salvianolicacidsodiuminterventioncansigniicantly increaseleftventricularejectionfractioneduceleftventricularend-diastolicandend-systolicdiameters，reversetheincreasnleft ventricularsortegatereaeuproxidsmutaseSioadialsdcreseatalase（Cndbitiof malondialdehyde（MDA），with all significantdifferences（ P<0.05） .Inthemodel group，the myocardial structure of ratswasdisordered，the arrangementofyoardalcellswsuorgadlembrofellswelldandosis，heifamatoyifiraiowaere andvacuolespresentedTeintervntionofsalvianolicacidsaltscouldsinificantlyimprovethearrangementofmyocardialcelsats， reducecelswlndrosslatefmmaollfiraiondipromntfaditf dose.ThemRAndproteinexpreiosofGCaspe-1，Vinterleukin（IL）-1βandIL18intemyoadfra inthemodel group significantly increased（ ⌈P<0.05⌉ ，and the intervention of salvianolic acid salts down-regulated the mRNA and protein expresinsofthabepathaysConcusioSavianolicacidsaltsanaleviatedoxorubciniducdmyocardaltoxcitydate the MAVS-NRP3-GSDMD signalingpathway，aleviate myocardialcellpyroptosis，andexertscardioprotectiveefects.

Keywordscellpyroptosis；myocardialtoxicitysavinolicacidsalts；mitocondrialantiviralsignalinprotein;NODikeecetorpin domain-containing protein 3; Caspase-1; experimental study

阿霉素具有广谱抗癌作用，是多种恶性肿瘤的常用化疗药物，可以有效改善病人的生存结局。（剩余9679字）