烟苗未知病害病际微生物菌群结构与多样性特征

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中图分类号：S432.4 文献标识码：A文章编号：2097-1354（2025）03-0034-09

开放科学（资源服务）标识码（OSID）：

Microbial Community Structure and Diversity Characteristics with an Unknown Disease of Tobacco Seedling

TANG Mei 1，2 ， BI Chaowei¹， LI Zhen²， SUN Meili²，³WANG Hancheng²， MENG Jianyu²， CAI Liuti²

1.Collegeof Plant Protection，Southwest University，Chongqing 40oooo，China；

2.Guizhou Academy of Tobacco Science，Guiyang 550081，China；

3.CollegeofAgriculture，YangtzeUniversity，Jingzhou，Hubei 434025，China

Abstract：An unknown tobacco seedling disease was found in Weining，Guizhou，mainly manifested as stem base constriction and root abscission，resulting in tobacco seedling wilting and death. Ilumina amplicon sequencing showed that the most dominant fungal genus in the stem base of healthy and infected tobacco seedlings was Talaromyces. The dominant bacteria of healthy tobacco seedlings were Wolbachia and Enterococcus. The dominant bacterial genera of infected tobacco seedlings were Rahnellal and Pseudomonas. The Shannon diversity index of fungi and bacteria in the stem base of healthy tobacco seedlings was significantly higher than that of infected tobacco seedlings.The results of Ilumina amplicon sequencing pointed out the goal for the subsequent selective isolation of Rahnellal ， Pseudomonas ， Cystobasidium with significantly increased abundance from infected tobacco seedlings，as well as Talaromyces fungi with insignificant increase in abundance but reported to be pathogenic， and confirmation of pathogens according to the Koch's rule.

Key words： high-throughput sequencing; community structure; diversity; tobacco seedling

我国是世界上最大的烟叶生产国，漂浮育苗是主要的育苗方式。（剩余9811字）