miR-424-3p靶向SMAD7调控子宫内膜癌 增殖、侵袭机制研究

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[中图分类号]R173 [文献标识码]A [文章编号]1673—5293（2026）02—0016-09

Study on mechanism of miR-424-3p targeting SMAD7 in regulating proliferationandinvasionofendometrialcarcinoma

CUI Jinghong1，CHEN Yang²，ZHANG Chaoxia1

（1.Department of obstetrics，Changde Hospital，Xiangya School of Medicine，Central South University eFirstPeople'sHospitalofChangdeCity），HunanChangde415Ooo，China;2.TheEmergencyDepartment

The22th hospital ofChinesepeople's liberationarmyhospital，Qinghai Golmud816Oo，China）

[Abstract]ObjectiveToinvestigate whethermiR-424-3pregulates the proliferationandinvasionof endometrialcarcinoma（EC）by mediatingSMAD7，andtoexploretheregulatorymechanism.Methods Predictivebinding siteanalysisandgeneexpresion assessment wereconductedusing TargetScan dataand the GEPIA database.Cellproliferation，migration，andinvasion were evaluated through EdUstaining，wound healing，andTranswellassays.Theregulatoryefectsof miR-424-3pandSMADonECwere evaluated invivothrough tumortransplantation experiments.Results Validationconfirmedthebinding sitebetweenmiR-424-3p andSMAD7.KnockoutofmiR-424-3psignificantlyinhibitedtheinvasionandproliferationofHEC-1AandRL95-2cels，while promotingcellapoptosis.However，sh-SMAD7reversed theefectsofmiR-424-3ponthesecells.Additionall，sh-SMAD7could significantlyounteracttheefectsofmiR-424-3pihbtorsontheexpressionofepithelialmesenchyaltransition（EMrelated proteinsinHEC-1AandRL95-2cellines.mR-424-3pinhibitorscouldsignificantlyinhibit thegrowthoftransplantedtumorsbut sh-SMAD7couldreversetheefectsofmiR-424-3pinhibitors.ConclusinmiR-424-3pregulates theproliferation，invasion，nd epithelialmesenchymal transition process of HEC-1AandRL95-2celsby modulating SMAD7，suggesting that miR-424-3p/ SMAD7 may be a new target for the prevention and treatment of endometrial carcinoma.

[Key words] miR-424-3p;endometrial carcinoma;SMAD7;epithelial-mesenchymal transition;proliferation;invasion

在全球，子宫内膜癌（endometrialcarcinoma，EC）的患病率呈上升趋势，尤其是在发达国家[1-2]根据世界卫生组织（WorldHealth Organization，WHO）统计，子宫内膜癌是全球女性生殖系统癌症中患病率最高的疾病之一[3]。（剩余11226字）