黄芩汤改善禽致病性大肠埃希菌感染雏鸡肠道屏障功能的机制

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Mechanism Skullcap decoction in improving intestinal barrier function in chicks infected with avian pathogenic Escherichia coli

Abstract： 【Objective】 To elucidate the underlying mechanisms by which the traditional Chinese medicinal formula Skullcap decoction （SCD） enhances intestinal barrier function in chicks challenged with avian pathogenic Escherichia coli （APEC）. 【Method】 An APEC infection model was established using male Isa

Brown chicks. The chicks were orally administered 0.05% （ω） SCD granules or modified formulations lacking a single herbal component：Scutellaria baicalensis （HQ），Paeonia alba （SY），Ziziphusjujuba（DZ） or Glycyiza uralensis （GC）. Serum diamine oxidase （DAO） level，and expression levels jejunal secretory immunoglobulin A （sIgA） and the tight junction proteins ZO-1 and Ocudin chicks were measured using ELISA. The mRNA expression levels ZO-1 and Ocudin in the jejunal mucosa were quantified by RT-qPCR. Gut microbial composition was analyzed through 16S rDNA sequencing，and the correlations between intestinal barrier function and microbial taxa were evaluated using Spearman correlation analysis. 【Result】 SCD administration significantly reduced serum DAO and jejunal sIgA levels （P<0.05） ，and upregulated ZO-1 and Occludin expression （P<0.05） in APEC-challenged chicks， indicating enhanced intestinal barrier function and maintenance gut microbial composition in APEC-challenged chicks.HQ exhibited multi-target regulatory activity on DAO sIgA and tight junction proteins， which was closely associated with gut microbiota modulation. DZ and SY exerted positive regulatory eects on expressions DAO and tight junction proteins， while GC showed a certain downregulatory effect on Occludin expression. 【Conclusion】 SCD enhances intestinal barrier function and maintains gut microbial homeostasis in APEC-chalenged chicks.Among its constituents， HQ serves as the core active component， while DZ， SY， and GC play synergistic auxiliary roles.

Keywords： Skullcap decoction; APEC; Chicken; Intestinal barrier; Gut microbiota

鸡大肠埃希菌病是由禽致病性大肠埃希菌（AvianpathogenicEscherichiacoli，APEC）感染引发的传染性细菌病，临床表现为局部或系统性的炎症反应，可导致腹膜炎、输卵管炎、卵黄囊感染、心包炎、气囊炎及败血症等，具有较高的发病率和死亡率，是禽类细菌性疾病中最常见、经济损失最大的疾病之一[1-2]。（剩余20700字）