肝细胞癌上皮细胞特征基因鉴定及其分子机制研究

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[中图分类号]R735.7 [文献标志码]A [DOI]10.19767/j.cnki.32-1412.2025.06.004

Identification of characteristic genes in hepatocellular carcinoma epithelial cells and investigationoftheirmolecularmechanisms

LI Ming

（DepartmentofLaboratoryMedicine，AffiliatedHospitalofNantongUniversity，Jiangsu226001）

[Abstract]Objective：Based on previous single-cellRNA sequencing （scRNA-seq） data revealing the high heterogeneityof hepatocelllarcarcinoma （HCC），thisstudyaimed to identifyandvalidate keydifferentially expressdgenes （DEGs）inmalignant epithelialcels，inordertoexploretheirpotentialasbiomarkersortherapeutictargets.Methods：Using previously established single-cell transcriptomicdataof HCC，weconducted in-depthanalyses focusing onmalignant epithelialcellpopulationsandscreenedforsignificantlydiferentiallyexpressedgenesthroughbioinformaticsapproaches. The expresion levelsof selected candidate genes were subsequently validated in hepatocellar carcinoma cellines using quantitativereal-time polymerasechain reaction （qRT-PCR）.Results：The single-celldataanalysissuccessfully identified Neurotensin （NTS），fibrinogenbetachain（FGB），long-chainacyl-CoAsynthetase4（ACSL4），materallyexpresedene3 （MEG3），leptinreceptor（LEPR），and Janus kinase 3 （JAK3）askey genes with specifically high expression inmalignant epithelial celsoflivercancer.qRT-PCR validationresultswere highlyconsistent with thesequencingdata，confirming the aberrant expressionpatterns of these genes inHCCcellinesand pinpointingasubset of genes exhibiting stable dysregulationatboth transcriptionalandpost-transcriptional levels.Conclusion：Byintegrating single-cell transcriptomicanalysis with invitromolecularvalidation，thisstudyestablishedacompleteresearchloopfrom bioinformaticdiscoveryto experimentalconfirmation.Thevalidated DEGsprovideasolid molecular foundation forunderstanding thepathogenesisof HCC and ofer valuable evidence supporting their potential as diagnostic biomarkers or therapeutic targets.

[KeyWords]hepatocellularcarcinoma;epithelialcells;single-cellRNAsequencing;diferentiallyexpressdgenes; ）markers

肝细胞癌（hepatocellular carcinoma，HCC）是最常见的肝脏原发恶性肿瘤，尽管外科切除、靶向治疗和免疫疗法取得进步，但由于HCC高度异质性、频繁复发和耐药性，患者整体预后仍不令人满意-。（剩余8524字）