胃癌细胞中细胞骨架结构相关基因的表达及其在胃癌发生发展中的作用

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[中图分类号]R735.2 [文献标志码]A [DOI]10.19767/j.cnki.32-1412.2025.06.001

Expression of genes related to the cytoskeletal structure in gastric cancer cells and their role in the occurrence and development of gastric cancer

WUJiangrongl，CHENG Xiangyu1，XIA Yiming1，LIMing² （Medical School of NantongUniversity，Jiangsu 226001; ²Department of Laboratory Medicine，Affiliated Hospital of Nantong University）

[Abstract]Objective：To investigate theexpresion characteristicsof molecular biomarkers ingastriccancercells andtheir roles in the occurrence and progresson of gastric cancer.Methods：The expression levels of genes such as PLEC，FAT1，EZR，HMGA1，MISPandPLAURinnormal humangastricepithelialcelsandgastriccancercellsweredetermined using real-time quantitative PCR（qRT-PCR） technology.The expresionsof PLECand PLAUR genes in MKN-1 and SGC-790lcelswererespectivelysilenced bygene interference technology，andtheproliferationabilitiesofcels in the si-PLEC，si-PLAUR and si-NC groups were determined by CCK8 method.Results：qRT-PCRresults showed that compared with normal human gastricepithelial cells，the expressonsofPLEC，FAT1，EZR，HMGA1，MISPandPLAUR genes in gastric cancer cells were significantly upregulated （ P <0.05）.CCK8 experiment results showed that the cell reproductivecapacity in thesi-PLEC and si-PLAUR groups was significantly lower than that in the si-NC group （ P< （20 0.05）.The tumorvolume formed by gastriccancercelswith PLEC expresion interference was significantlysmaler than that ofthecontrol group，andthetumor growthrate was significantlyslower.Conclusion：PLEC，FAT1，EZR，HMGA1， MISPand PLAURplayimportantroles intheoccurrenceanddevelopmentof gastriccancer，andmayserveaspotential molecular biomarkers for early diagnosis and targeted treatment.

[Key Words] gastric cancer; molecular biomarkers; cell proliferation; tumor xenograft in nude mice

胃癌是全球最常见的消化系统恶性肿瘤之一，其发病率和死亡率居高不下，对人类健康构成严重威胁1。（剩余5651字）