水稻转录因子OsABF5基因的克隆及表达分析

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关键词水稻；OsABF5；基因克隆；非生物胁迫；表达分析

中图分类号S511文献标识码A

文章编号 0517-6611（2025）14-0110-06

doi：10.3969/j.issn.0517-6611.2025.14.022

开放科学（资源服务）标识码（OSID）：

Cloning and Expression Analysis of Transcription Factor OsABF5 Gene in Rice

N Chang，LONG Jiang-hui， HUANG Pei-ying et al（Guizhou Rice Research Institute，Guiyang，Guihzou ）

Abstract RT-PCR technology was used to obtain the complete cDNA sequence of the OsABF5 gene from the self-bred restorer line rice material“R1sidsatalisdepresioaracestcaliserbioticrssofeee total length of OsABF5 gene is 1023bp ，encoding 340 amino acids.The instability index of its encoded protein is 57.91，and the average hydrophilicity is -0.611 ，indicating that it is anunstable hydrophilic protein.Itpossesses bZIP46 domain and belongs tothebZIP superfamily， without transmembrane domains or signal peptides. It is distantlyrelated to OsABF1 inrice and most closely related to TdABF5 in Triticum dicocoides.ThepefOBisstld，istildspdo anther，leafdoExpresioracterstcalsodatieratu，tpatureouhtdalreli it the expression of the OsABF5 gene.

Keywords Rice;OsABF5;Gene cloning;Abiotic stress;Expression analysis

水稻是世界上最重要的谷类作物之一，是世界 50% 以上人口的主食[1]。（剩余10698字）