一株水稻促生贝莱斯芽孢杆菌20-10的高密度液体发酵技术研究

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关键词贝莱斯芽孢杆菌20-10;正交试验；响应面试验；高密度液体发酵；发酵条件优化

中图分类号S-3文献标识码A

文章编号0517-6611（2025）14-0009-07

doi：10.3969/j.issn.0517-6611.2025.14.002

开放科学（资源服务）标识码（OSID）：

dy on High Density Liquid Fermentation Technology of a Rice Growth Promoting Bacillus velezensis 20. YANG Hua，HU Zhan，WU Shan-dong et al（Hunan Institute of Microbiology，Changsha，Hunan 410009）

AbstractThesinglefactorandorthgonalexperimentswereusedtooptiizethecompostionandcontentofliqudculturemedforBacil lus velezensis 2O-1O，the optimal culture medium components were maltose 20.0g/L ，starch 10.0g/L ，yeast paste 12.5g/L ，NaCl 1.0g/L （2 （NH4）2SO43.0g/L . In this culture medium，the bacteria concentration was 45.5×108 CFU/mL，which increased by 106.82% compared to thebasicculturemdiufeeingcluredinstrain2O-10shakeflasksfor7hourseotialfermentationparametersobdbtimizing the fermentation conditions of 20-1Othrough single factor and response surface experiments were inoculation amount 2.0% ， temperature 30% ，and pH7.0 .Undertheseoptimized conditions，thebacterial concentrations obtained by expandingthe cultivation of the strains in 20L and 500L fermenters for 64 hours were 52.0×108 and 160.5×108 CFU/mL，respectively，which were 1.36 and 6.30 times higher than the basic culture medium.

KeywordscileesisOgalexpt;spseeepent;Hghsitydatio timization

贝莱斯芽孢杆菌（Bacillusvelezensis）是一种革兰氏阳性好氧的产芽孢杆菌，广泛分布在土壤、植物表面和根际[，因其在生长与繁殖过程中能够产生种类丰富的次级代谢产物，并且具有优秀的促植物生长和广谱而有效的抑菌特性被关注[2]。（剩余12648字）