血浆胞外囊泡LncRNATUG1在非小细胞肺癌中的表达及意义

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[关键词］LncRNATUG1;非小细胞肺癌;血浆;胞外囊泡;诊断标志物

［中图分类号］R734.2［文献标志码］A [文章编号] 1671-7783（2025）06-0461-07

[Abstract]Objective： To screen taurine upregulated gene 1（TUG1），a long non-coding RNA（LncRNA）with highly specific differential expression，in plasma extracellular vesicles （EVs） from patients with non-small cell lung cancer（NSCLC），and to validate its clinical value as a diagnostic biomarker for NSCLC. Methods： LncRNA sequencing was performed on plasma EVs from 6 NSCLC patients and 6 healthy individuals. Quantitative real-time reverse transcription polymerase chain reaction （qRT-PCR） was used to detect the expression of LncRNA TUG1 in plasma EVs from 6O NSCLC patients，6O healthy controls，and 93 patients for differential diagnosis （including small cellung cancer， mediastinal lymphoma，etc.）.The correlation between TUG1 expression and clinical characteristics of NSCLC was analyzed.The effect of TUG1 on the biological behaviorof NSCLC cels was explored through in vitro celular experiments，and the receiver operating characteristic （ROC）curve was used to evaluate its diagnostic efficacy. Results： Sequencing and qRT-PCR verification showed that TUG1 expression in plasma EVs of NSCLC patients was significantly higher than that in healthy individuals （ P<0.01 ），and its expression in multiple NSCLC cellines was higher than that in normal bronchial epithelial cells. Notably，TUG1 expression in plasma EVs of NSCLC patients decreased significantly after surgery （ P< 0.01）.TUG1 expression was correlated with tumor size，distant metastasis，lymph node metastasis，and TNM stage （ P<0.05 or P<0.01 ）.Knockdown of TUG1 significantly inhibited the proliferation and migration abilities of H1299 cells （ P<0.05 or P<0.01 ）. ROC curve analysis showed that the area under the curve （AUC）of TUG1 for diagnosing NSCLC was O.849 （specificity =0.83 ，sensitivity = 0.86）. When combined with carcino-embryonic antigen（CEA），the AUC was further improved to 0.882（ P< 0.01）.Conclusion： LncRNA TUG1 is highly expressed in plasma EVs of NSCLC patients and closely associated with tumor progression.It can serve as a novel diagnostic biomarker for NSCLC，and combined detection with CEA can enhance diagnostic efficacy.

[Key Words]LncRNA TUG1; non-small cell lung cancer （NSCLC）； plasma; extracellular vesicles ;diagnostic biomarker

肺癌是全球发病率和死亡率均居首位的恶性肿瘤[1]，主要分为小细胞肺癌（small cell lungcancer，SCLC）和非小细胞肺癌（non-small cell lung cancer，NSCLC）两大类。（剩余11056字）