双联益生菌水凝胶的制备及其选择性抑菌和清除活性氧效果评价

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Synthesis of a dual-probiotic hydrogel and evaluation of its selective antibacterial and reactive oxygen species scavenging effects

CHEN Jialing， HUANG Yuwen， LU Kecheng， HE Xueting， ZHANG Maxuyang， SHEN Haijun（School of Medicine， Jiangsu University， Zhenjiang Jiangsu 212O13， China）

[Abstract] Objective： To develop a wound dressing incorporating Clostridium butyricum （C. butyricum， anaerobic probiotic），which is capable of producing antibacterial substances and exerting hydrogenproducing properties under normoxic conditions，and investigate the efects of its selective bacteria inhibition and reactive oxygen species （ROS） scavenging. Methods： C. butyricum and Bacillus subtilis （B. subtilis，aerobic probiotic） were simultaneously encapsulated in hydrogel to prepare a dualprobiotic hydrogel （ BC -gel）. B. subtilis was used to consume oxygen within the hydrogel. This creates an anaerobic microenvironment for C . butyricum to maintain its viability. The selective bacteria inhibition of BC -gel was evaluated by plate counting method，while the selective scavenging of ROS was evaluated through flow cytometry. Results： BC -gel can effectively inhibit the proliferation of Escherichia coli and methicilln-resistant Staphylococcus aureus. While maintaining the normal growth of Bifidobacterium and Lactobacillus ， BC -gel has the ability to scavenge ·OH and ONOO free radicals， but has no significant decreasing effect on O2- ·and NO·. Conclusion： BC -gel exhibits favorable selective bacteria inhibition and ROS scavenging properties.

[Key words]diabetic ulcer； wound repair； Clostridium butyricum； Bacillus subtilis；antibacterial;reactive oxygen species; dual-probiotic hydrogel

随着生活质量的提升，糖尿病发病率显著增加，已成为全球性公共卫生问题。（剩余10162字）