【摘要】 目的 观察葛根素预处理对大鼠肝脏缺血再灌注损伤的防护作用。方法 雄性SD大鼠，建立肝脏缺血再灌注模型(HIR)。随机分为假手术组、HIR组和HIR-葛根素预处理组。黄嘌呤氧化酶法、硫代巴比妥酸比色法分别测定肝组织中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性的变化，同时分析NO含量的改变。结果 肝脏缺血再灌注损伤后，与假手术组比较，肝组织中MDA活性及NO含量均显著增加，而SOD活性显著降低；经40 mg/kg剂量的葛根素预处理7 d后，与模型组相比，肝组织中MDA活性及NO含量显著降低，而SOD活性增高。结论 葛根素预处理对大鼠缺血再灌注肝脏损伤有一定的保护作用。
【关键词】 葛根素；缺血再灌注；肝脏；抗氧化
The antioxidation effect of kakkonein on hepatic ischemia reperfusion injury in rats
LUO Guang-qiang,Xi’an 630 Hospital, Xi’an, Shanxi 710000,China
【Abstract】 Objective To investigate the antioxidation effect of kakkonein on hepatic ischemia reperfusion injury in rats． Methods Male Sprague-Dawley rats were randomly divided into three groups: pseudo-operation normal control group， hepatic ischemia reperfusion injury model group, kakkonein group. After hepatic ischemia and reperfusion, to analysis the level of MDA, SOD and NO activity. Results After hepatic ischemia reperfusion, the MDA and NO level increased, and the SOD activity decreased obviously comparing with the normal group; Comparing to the hepatic ischemia reperfusion injury model group, the MDA and NO level decreased, and the SOD activity increased. Conclusions The apigenin have a prominent protective effect on hepatic ischemia reperfusion injury in rats．
【Key words】Kakkonein; Ischemia reperfusion; Liver; Antioxidation
肝脏缺血再灌注损伤是肝脏缺血后重获血流灌注或氧供后，引起或加重肝脏缺血缺氧造成的损伤，是临床肝脏手术过程中常见的一种病理生理变化，可产生氧自由基，引起钙超载等改变，损伤肝细胞超微结构，影响肝脏的功能[1]。（剩余1558字）