【摘要】 目的通过免疫组化方法对乳腺增生病、乳腺不典型增生及乳腺癌进行多指标联合检测，并结合显微镜下组织学形态特点进行观察，以利于乳腺癌早期发现及早期诊断。方法 采用免疫组化方法检测ck/34BE12、actin、P63、S-100、C-erbB-2、PCNA、CyclinD1在159例乳腺疾病组织中的表达。结果 ck/34BE12、P63、actin在乳腺良性病变中高表达，在恶性病变中低表达呈阴性，S-100差异不显著（P>0．05）, C-erbB-2、PCNA、CyclinD1在乳腺增生病中呈阴性表达，随着上皮增生和异型程度加重，其阳性表达逐渐递增，其中C-erbB-2在原位癌达高峰，而侵润性癌则出现下降，结论 对乳腺疾病特别是乳腺不典型增生及乳头状瘤病进行ck/34BE12、actin、P63、S-100、C-erbB-2、PCNA、CyclinD等多项指标检测有利于早期发现乳腺癌，并将为乳腺癌的病理学早期诊断提供新的重要依据。
【关键词】乳腺增生病；乳腺非典型增生；乳腺癌
Expression of ck/34BE12，actin，P63，S-100，C-erbB-2，PCNA，CyclinD1 genes in breast epithliosis and breast cancer
CAI Bo-yu,ZHU Ying． Department of Pathology,The first Hospital in Qiqihar City, Heilongjiang161006 ,China
【Abstract】 ObjectiveImmunohistochemical reaction were evaluated in benign epithelial proliferations,atypical hyperplasia of breast and breast cancer as well as observation with microscope In order that it will be help us identifying benign epithelial proliferations and carcinomas of the breast ．Methods 159 cases of breast diseas tissues were detected the expression of ck/34BE12,actin,P63 S-100,CyclinD1,PCNA,C-erbB-2 by immunohistochemicalResultsThe positive rates of ck/34BE12,P63 actin in the MECS were expressed highly in benign epithelial, except the group of m alignant breast carcinoma.The positive rates of S-100 were low in each group The positive rates of C-erbB-2,PCNA,CyclinD1 were improved gradually with light atypical hyperplasia of breast -heavy atypical hyperplasia of breast-breast cancer． Conclusionck/34BE12,actin,P63,CyclinD1,PCNA,C-erbB-2 multiple parameter detection as well as observation with HE stain are usofu for indentification of benigr and m alignant epithelial lesions of the breast．
【Key words】Breast carcinoma epithliosis; Benign epithelial proliferation ；Breast cancer
乳腺和其他器官一样，按照肿瘤的发生发展规律，乳腺癌也必然经过：乳腺增生病-乳腺非典型增生-原位癌-侵润性癌这一过程，其中乳腺非典型增生为癌前病变，与原位癌的鉴别诊断在显微镜下非常困难[1]，因此笔者尝试着用免疫组化方法，对乳腺癌及其癌前病变进行多指标联合检测，以利于乳腺癌早期发现及早期诊断。（剩余1193字）