【摘要】 目的 建立同时测定复方丹参滴丸中丹参素和原儿茶醛的高效液相色谱法。方法 选用C18柱，流动相为甲醇-水-冰醋酸(19∶80∶1)，检测波长为279 nm。 结果 丹参素和原儿茶醛分别在10~80 mg/L和2~16 mg/L线性范围关系良好,丹参素和原儿茶醛的平均回收率分别为99．4%和99．5%，RSD分别为1．6%和1．5%。结论 方法简便、灵敏、准确，样品处理简便易行，可作为复方丹参滴丸的质量控制方法。
【关键词】 复方丹参滴丸；丹参素；原儿茶醛；高效液相色谱法
Analysis of Danshensu and Protocatechuic Aldehyde in Compound Danshen Dropping Pill by High Performance Liquid Chromatoraphy
DING Lan，YI Ai-chun，SHI Zheng-rong.The First Hospital of Changsha，Changsha 410005，China
【Abstract】 Objective To separate and determine Dunshensu and protocatechuic aldehyde in Compound Danshen Dropping Pill.Methods The chromatographic conditions were as follow:Nucleosil C18 column.A mixture of methanol-water-acetic acid glacial(19∶80∶1)as the mobile phase with a flow rate of 1 ml per minute and the detection wavelength at 279 nm.Phydroxybenoic acid was used as internal standard.Results The calibration curves were linear in the range of 10~80 mg/L for Danshensu and 2~16 mg/L for protocatechuic aldehyde.The average recovery of Danshensu and protocatechuic aldehyde were 99．4%and 99．5%，and RSD were 1．6%and 1．5%.Conclusion This method is accurate，simple and convenient,rapid and reproducible and can be used for the quality control of compound Danshen Dropping Pill.
【Key words】 Compound Danshen dropping pill； Danshensu； Protocatechuic Aldehyde；HPLC
复方丹参滴丸(Compound Danshen Dripping Pills)是根据中国药典1995年版一部复方丹参片的处方，在现代高科技条件下提取丹参、三七的有效成分再加入适量冰片而制成的新型纯中药滴丸剂，其药理作用为活血化瘀，理气止痛，用于气滞血瘀所致的胸痛，心前区刺痛，临床上用于治疗胸中憋闷、心绞痛。（剩余2310字）