摘要：以热产硫化氢高温厌氧杆菌菌液为模板，根据Gene Bank中登记的编码结构域Tt APuX21基因序列设计1对特异引物，利用多聚酶链反应技术，扩增出目的基因Tt apux21，并克隆到表达载体pET21a(+)中。经菌液PCR筛选和DNA测序鉴定，表达载体pET21a(+)中插入有序列正确的Tt apux21基因，重组质粒命名为pEX21。
关键词：结构域；PCR扩增；克隆
中图分类号：Q78 文献标识码：A 文章编号：0439-8114(2008)05-0493-03
Study on Isolation and Cloning of the Gene Encoding Module Tt APuX21
XIE Wan-bin
(College of Chemistry and Bioengineering，Yichun University，Yichun 336000，Jiangxi，China)
Abstract：A pair of specific primers were designed according to the gene sequence encoding module Tt APuX21 adopted in Gene Bank. By the improved PCR amplification technology，interest gene Tt apux21 was obtained from Thermoanaerobacter thermohydrosulfuricus，and then cloned into expression vector pET21a(+). Bacterial culture PCR showed that interest gene Tt apux21 had been inserted into pET21a(+)，and DNA sequencing revealed that the sequence of the inserted fragment was the same as that of Tt apux21 adopted in Gene Bank (accession number：M28471). The recombinant plasmid was designated as pEX21.
Key words：module；PCR amplification；cloning
能源短缺已成为世界范围内普遍存在的问题，且随着石油、天然气等不可再生能源的消耗速度加快，能源短缺问题越来越严重。（剩余2101字）